临床神经外科杂志2017，Vol.14Issue(5)：385-389,5.DOI:10.3969/j.issn.1672-7770.2017.05.016
悬浮培养与贴壁培养方式神经干细胞分化表型特点
Neural stem cells and phenotypic characteristics of suspension culture and adherent culture
刘友 1刘希光 1李爱民1
作者信息
- 1. 222002 连云港,徐州医科大学附属连云港医院神经外科
- 折叠
摘要
Abstract
Objective To observe the morphological characteristics and differentiation phenotype of neural stem cells in suspension culture and adherent culture .Methods The neural stem cells ( NSCs) of rat cortex was cultured by serum-free suspension culture and adherent culture . The morphological characteristics of neural stem cells were observed .In vitro fetal bovine serum (FBS) induced differentiation of NSCs,and differentiated phenotypes were identified by Nestin and GFAP immunofluorescence staining .Proven blue viable cell count method compared cell proliferation ability .Results The neonatal rat cortex was cultured in serum-free suspension to obtain stable NSCs cell pellets .Adherent culture was used to form long fusiform NSCs .NSCs were positive for nestin immunofluorescence staining .In vitro, FBS induced differentiation , radial nerve fibers began to appear in the nerve ball , immunofluorescence staining showed GFAP positive cells outward and Nestin positive cells set .NSCs were stained into astrocytes , and Nestin and GFAP staining positive cells were observed by immunofluorescence staining .The proliferation rate of the neural stem cells cultured in the same way was the highest at the 4th ~3rd day,and the proliferation rate was the highest at the 4th ~5th day.Conclusions The neural stem cells can be successfully cultured in vitro by suspension culture and adherent culture using the serum -free culture medium B27 and bFGF,EGF combined neural stem cells .Cultivation of neural stem cells by suspension culture was superior to adherent culture in early proliferation rate .关键词
神经干细胞/悬浮培养/贴壁培养Key words
neural stem cells/suspension culture/adherent culture分类
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刘友,刘希光,李爱民..悬浮培养与贴壁培养方式神经干细胞分化表型特点[J].临床神经外科杂志,2017,14(5):385-389,5.
