环境与职业医学2017，Vol.34Issue(9)：836-839,4.DOI:10.13213/j.cnki.jeom.2017.17129
超高效液相色谱法测定大气PM2.5中16种多环芳烃
Determination of polycyclic aromatic hydrocarbons in PM2.5 by ultrasensitive performance liquid chromatography
张艳 1冯翠霞 1陈剑刚1
作者信息
- 1. 珠海市疾病预防控制中心理化检验所,广东珠海519060
- 折叠
摘要
Abstract
[Objective] To develop a method for the determination of polycyclic aromatic hydrocarbons (PAHs) in atmospheric fine particulate matters (PM2.5) by ultrasensitive performance liquid chromatography.[Methods] Samples were extracted and enriched with acetonitrile by ultrasonic wave.Then acetonitrile and water were used as mobile phase for gradient elution,and separation was conducted on a PAH C18 column.Sample preparation and chromatographic conditions were optimized.Ultrasensitive performance liquid chromatography,photodiode array detector,and fluorescence detector were used to detect PAHs.[Results] Sixteen PAHs were separated in 12 min,showing a good linearity in the range of 0.01-0.50 μg/mL.The limits of detection for the PAHs were 0.003-0.036 ng/m3,and the limits of quantification were 0.010-0.119 ng/m3.The average recoveries of 16 PAHs at 0.02,0.05,and 0.20μg spiked levels were 68.5％-100.6％,with relative standard deviations of 2.0％-7.3％ (n=7).[Gonclusion] The method developed is simple,rapid,efficient,and sensitive;therefore,it is suitable for determination of PAHs in PM2.5.关键词
超高效液相色谱/PM2.5/多环芳烃Key words
ultrasensitive performance liquid chromatography/PM2.5/polycyclic aromatic hydrocarbon分类
医药卫生引用本文复制引用
张艳,冯翠霞,陈剑刚..超高效液相色谱法测定大气PM2.5中16种多环芳烃[J].环境与职业医学,2017,34(9):836-839,4.
