林业科学2017，Vol.53Issue(8)：43-53,11.DOI:10.11707/j.1001-7488.20170806
湖南省油茶炭疽病病原鉴定
Pathogen of Oil-Tea Trees Anthracnose Caused by Colletotrichum spp. in Hunan Province
摘要
Abstract
[Objective]The filamentous fungi,Colletotrichum spp.,are found in all five continents and are capable of causing severe diseases in the important plants such as Camellia oleifera (oil-tea trees). The pathogen causes defoliation, as well as necrotic spots on fruits. In recent years,the disease is rapidly spreading in the oil-tea trees producing areas of southern China. The objective of this study is to identify the pathogen causing stem and leaf anthracnose on oil-tea trees in Hunan,and to provide a basis for disease control and resistance breeding. [Method]Infected leaves were collected from Changsha,Zhuzhou,Liuyang,Yongzhou,Huaihua,Changde and Changning City,in Hunan Province and the fungi were isolated and purified from the diseased border of leaves on acidified potato dextrose agar(PDA) plate. Identification of the pathogen was based on morphology of colony and conidial,pathogenicity tests. Isolates were characterized by colony morphology,conidial characteristics under 28 ℃ in dark. The rDNA-ITS,CAL and GAPDH region of isolates were amplified and sequenced. PAUP and MrBayes software were used to analyze the data of ITS-CAL-GAPDH sequences.[Result]The result showed that a total of 62 colletotrichum strains were isolated from oil tea plants in 9 oil-tea plantations of Hunan provinces. Pathogenicity test showed that all of the isolates could infect oil-tea leaves and fruit,with different time in the onset of disease symptom. The multilocus sequence analysis,together with a critical examination of the phenotypic characters,revealed five previously described species. [Conclusion]The typical anthracnose symptoms of oil-tea leaves in Hunan Province were described in this study. Based on the morphology and multiple gene sequences characters,the 62 isolates were identified as C. fructicola,C. siamense,C. gloeosporioides,C. horii and C. camelliae. Among them,C. fructicola had the highest isolated rate that was accounted for 64.5%.关键词
油茶/炭疽病/病原菌Key words
oil tea trees/anthracnose/pathogen分类
农业科技引用本文复制引用
李河,李杨,蒋仕强,刘君昂,周国英..湖南省油茶炭疽病病原鉴定[J].林业科学,2017,53(8):43-53,11.基金项目
国家自然科学基金项目(31570641) （31570641）
教育部农作物生物灾害综合治理重点实验室/农业部华东作物有害生物综合治理重点实验室开放课题. （）
