林业科学2017，Vol.53Issue(8)：141-148,8.DOI:10.11707/j.1001-7488.20170816
针叶小爪螨不同种群内共生菌Wolbachia和Cardinium的检测及系统发育分析
Detection of Wolbachia and Cardinium Endosymbionts in Different Oligonychus ununguis Populations and Phylogenetic Analysis of Cardinium
摘要
Abstract
[Objective] There exists reproductive incompatibility between the Castanea mollissima population from Shandong Province (TSBL) and the Cunninghamia lanceolata population from Zhejiang Province (ZJSM) of Oligonychus ununguis. However,Wolbachia or Cardinium,as the insect's endosymbiont,has the ability to alter the reproductive capabilities of its host. This study aims to analyze the distribution of Wolbachia and Cardinium in different O. ununguis populations,and to reveal the effects of endosymbionts on O. ununguis reproductive incompatibility.[Method]Primers were designed according to the sequences of the gene encoding a surface protein of Wolbachia (wsp) and the Cardinium's 16S rRNA gene,and PCR was performed using the genomic DNA extracted from a single adult female O. ununguis. Purified PCR fragments were sequenced and blasted against the databases. Phylogenetic tree was constructed based on these sequences.[Result]Neither the C. mollissima population from Shandong Province (TSBL) nor the C. lanceolata population from Zhejiang Province (ZJSM) was infected with Wolbachia. In all the detected 12 populations of O. ununguis,the infection rates of Wolbachia and Cardinium were 0 and 8.3%,respectively. Cardinium was detected only in the Quercus acutissima population from Hebei Province,but the intrapopulation infection rate was high with 66.7%.The Cardinium infecting O. ununguis was closer to that from Teranychus urticae in phylogenetic relationship.[Conclusion]The infection rate of Wolbachia might be very low in O. ununguis in China. The reproductive incompatibility between the C. mollissima population from Shandong Province (TSBL) and the C. lanceolata population from Zhejiang Province (ZJSM) of O. ununguis was independent of Wolbachia and Cardinium.关键词
针叶小爪螨/种群分化/Wolbachia/Cardinium/系统发育Key words
Oligonychus ununguis/population differentiation/Wolbachia/Cardinium/phylogeny分类
农业科技引用本文复制引用
孙菲,杨春红,李波,郑金柱,周成刚,谢丽霞,尹淑艳..针叶小爪螨不同种群内共生菌Wolbachia和Cardinium的检测及系统发育分析[J].林业科学,2017,53(8):141-148,8.基金项目
山东省高等学校科技计划项目(J14LE14) （J14LE14）
国家自然科学基金项目(31401957). （31401957）
