南京农业大学学报2017，Vol.40Issue(5)：795-803,9.DOI:10.7685/jnau.201612040
基于matK基因和ITS序列的江苏地方豆类植物的亲缘关系研究
Phylogenetic relationship among local legumes in Jiangsu Province based on analyses of matK gene and ITS sequence
摘要
Abstract
[Objectives] Genetic relationships among 14 legumes were analyzed under the molecular level,in order to provide a theoretical basis for the germplasm innovation and genetic improvement in legumes.[Methods] We sequenced the matK gene and ITS sequence of 14 Jiangsu local varieties of legumes collected by our laboratory and another four legumes (Glycine max,Phaseolus vulgaris,Vigna angularis and Vigna radiata)from GenBank.Then we compared and analyzed the matK gene and ITS sequences of 18 legumes by Clustal W,MEGA 6.0 and BioEdit.A phylogenetic tree was constructed and the divergence time was also calculated by using Lotus corniculatus as outgroup.[Results] The results showed that the length of matK gene in 18 legumes was 750 bp after alignment,including 85 variable sites and 82 informative sites with the genetic distance that ranged from 0 to 0.099.The length of ITS sequence was 795 bp after alignment,including 260 variable sites and 242 informative sites with the genetic distance that ranged from 0 to 0.292.The phylogeuetic tree constructed by matK gene and ITS sequence showed that 18 legumes were divided into three groups,Vigna angularis and Vigna radiate were grouped to one branch,Glycine max and Phaseolus vulgaris were grouped into the other two branches,respectively.The estimated divergence times were 19.02 million years ago(Glycine max vs Phaseolus vulgaris),13.50 million years ago(Phaseolus vulgaris vs Vigna angularis)and 2.94 million years ago(Vigna radiate vs Vigna angularis)for the three respective groups.[Conclusions] Sequence analyses and results from phylogenetic tree were consistent with the morphologic identification in these legumes,which suggested that both matK gene and ITS sequence could be used for phylogenetic analysis among species in local legumes.关键词
豆类植物/matK基因/ITS序列/亲缘关系/分化时间Key words
legumes/matK gene/ITS sequence/phylogenetic relationship/divergence time分类
农业科技引用本文复制引用
王彤,刘静,郭月,袁娜,杜建厂..基于matK基因和ITS序列的江苏地方豆类植物的亲缘关系研究[J].南京农业大学学报,2017,40(5):795-803,9.基金项目
国家自然科学基金项目(31370266) （31370266）
江苏省农业科技自主创新基金项目[CX14(5010)] （5010）
