山西医科大学学报2017，Vol.48Issue(9)：895-899,5.DOI:10.13753/j.issn.1007-6611.2017.09.006
活细胞内基于Diels-Alder反应的量子点标记蛋白质研究
Protein labeling with quantum dots in live cells based on Diels-Alder reactions
摘要
Abstract
Objective To establish a new method of labeling a protein with quantum dots (QDs) within live cells via the Diels-Alder cycloaddition.Methods Transcription activator-like effectors(TALE) decorated with trans-cyclooctene TCO2 and tetrazine 1 (Tzl)-conjugated QD625 were combined specially in the live Vero cells to yield a QD625-labelled TALE(TALE-QD625).The distribution of fluorescent signal for TALE-QD625 in Vero cells were observed through confocal microscopy.Results The QD625 was conjugated with TALE based on the Tzl and TCO2 Diels-Alder cycloaddition labeling system and subsequently entered the cell nucleus.Red fluorescent signal of TALE-QD625 was observed in the nucleus of Vero cells.Conclusion It is the first time that QDs have been used to specially label a protein via the Diels-Alder cycloaddition within live cells,which will provide a new labeling method for single protein molecule imaging.关键词
蛋白质标记/量子点/Diels-Alder环加成反应/活细胞/荧光纳米材料Key words
protein labeling/QDs/Diels-Alder cycloaddition/live ceil/fluorescent nanomaterial分类
医药卫生引用本文复制引用
王明秀,张加玲,刘云凤,乔增杰..活细胞内基于Diels-Alder反应的量子点标记蛋白质研究[J].山西医科大学学报,2017,48(9):895-899,5.基金项目
病毒学国家重点实验室开放研究基金资助项目(2017IOV005) （2017IOV005）
山西医科大学青年基金资助项目(02201512) （02201512）
