山西医科大学学报2017，Vol.48Issue(9)：918-921,4.DOI:10.13753/j.issn.1007-6611.2017.09.011
γ-分泌酶抑制剂在哮喘小鼠模型Th17/Treg平衡失调中的作用
Role of γ-secretase inhibitor in the imbalance of Th17/Treg in a mouse asthmatic model
摘要
Abstract
Objective To explore the effects of γ,-secretase inhibitor(DAPT) on the airway inflammation,the airway hyperactivity and the imbalance of Thl7/Treg in a mouse model of allergic asthma for providing a theoretical basis for the research and development of anti-asthma activity preparations.Methods Forty-five female C57BL/6 mice were randomly divided into 3 groups:control group,asthma group and DAPT group.The pathological changes of lung tissue were analyzed by hematoxylin-eosin staining.Levels of cytokine and inflammatory mediators in bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay.The expression of Foxp3 mRNA in spleen was detected by reverse transcription polymerase chain reaction(RT-PCR).Results The airway inflammation and mucus secretion were alleviated in DAPT group compared with asthma group.The contents of IgE,IL-17,IL-I0 and TGF-β1 in asthma group were (695 + 148) ng/ml,(58 + 6) pg/ml,(148 + 12) pg/ml,(18-+ 3) pg/ml,respectively.The contents of IgE,IL-17,IL-10 and TGF-131 in DAPT group were (486 + 152) ng/ml,(27 ± 6) pg/ml,(193 + 17) pg/ml,(33 ± 5) pg/ml,respectively.Compared with asthma group,the levels of IgE and IL-17 were significantly decreased in DAPT group(P ＜ 0.01),the levels of IL-10,TGF-β1 and the expression of Foxp3 mRNA were significantly increased in DAPT group (P ＜ 0.01).Conclusion The γ-secre tase inhibitor DAPT may efiqciently regulate the imbalance of Th17/Treg to balance a mouse asthmatic model.关键词
支气管哮喘/Notch信号/γ-分泌酶抑制剂/Th17/Treg失衡/小鼠Key words
asthma/Notch signal/γ-secretase inhibitor/imbalance of Th17/Treg/mice分类
医药卫生引用本文复制引用
辛娜,李敬梅,王永锋,王瑶,陈婕,杨超,吴跃刚..γ-分泌酶抑制剂在哮喘小鼠模型Th17/Treg平衡失调中的作用[J].山西医科大学学报,2017,48(9):918-921,4.基金项目
陕西省教育厅专项科研计划项目(16JK1647) （16JK1647）
