畜牧与兽医2017，Vol.49Issue(10)：74-80,7.
猪流行性腹泻病毒广东分离株GD/JMEP的遗传演化分析
Analysis of genetic evolution of porcine epidemic diarrhea virus GD/JMEP isolated from Guangdong Province
摘要
Abstract
This study aimed to investigate the epidemic situation of porcine epidemic diarrhea virus (PEDV).One PEDV strain named GD/JMEP was isolated.The S,N,M and ORF3 genes of the isolate were cloned and sequenced as well as analyzed by bioinformatics.The results indicated that the tested PEDV strain GD/JMEP was far from CV777 strain but had closer relationship with the epidemic PEDV strains in Guangdong,which were in the same branch.No deficiency of acid sequence was found in the ORF3 gene.Nucleotide and amino acid sequence analysis of N or M gene indicated GD/JMEP exhibited fewer identities compared with classical strain such as EAS1,but a high identity with the epidemic strains in China,American and Korea from 2013 to 2015.Nine amino acid mutation in N gene and 11 nucleotide and 3 amino acid mutations in M gene were detected in GD/JMEP isolate compared with classical CV777.Nucleotides insertion or deletion of GD/ JMEP were found in several sites of S gene compared with classical strains.New mutation at 406nt of S gene compared with the latest epidemic strain was founded.Six new mutations of amino acid sites indicated GD/JMEP was a variant epidemic strain in Guangdong Province.关键词
猪流行性腹泻病毒/遗传变异/S基因/N基因/M基因Key words
porcine epidemic diarrhea virus/genetic variation/S gene/N gene/M gene分类
农业科技引用本文复制引用
周如月,刘琪,贾爱卿,王贵平..猪流行性腹泻病毒广东分离株GD/JMEP的遗传演化分析[J].畜牧与兽医,2017,49(10):74-80,7.基金项目
广州市产学研协同创新重大专项(201508020039) （201508020039）
广州市南沙区科技计划项目(2014KF01) （2014KF01）
