眼科新进展2017，Vol.37Issue(10)：906-910,5.DOI:10.13389/j.cnki.rao.2017.0230
高钙状态对人晶状体上皮细胞株SRA01/04氧化应激水平的影响
Effects of calcium elevation on intracellular oxidative stress in human lens epithelial cells SRA01/04
摘要
Abstract
Objective To investigate the influence of calcium elevation on oxida tive stress in human lens epithelial cells (HLEC) SRA01/04.Method The cells (2 x 103 cells/well) which in the period of logarithmic phase were seeded into 96-well plates with three replicates for the two groups;and in the experimental group,SRA01/04 cells were exposed to a CaCI2 concentration gradient (3 mmol · L-1,5 mmol · L-1,7 mmol · L-1,9 mmol · L-1,11 mmol · L-1,13 mmol · L-1,15 mmol · L-1,17 mmol · L-1,19 mmol · L-1) for 0 h,12 h,24 h,36 h;while the cells in the control group were cultured in complete 1640 medium.Cell counting kit-8 (CCK-8) assay was used to measure cell viability.The levels of intracellular superoxide dismutase (SOD),glutathione (GSH) content and oxidized glutathione (GSSG) / total glutathione (T-GSH) were determined by using the microplate-reader method with the commercial total/oxidized glutathione and sod quantification kit.Results At first,the survival rate of SRA01/04 cells treated with 3 mmol · L-1,5 mmol · L-1,7 mmol· L-1 CaCL2 for 24 h showed a significant decrease with the increase of CaCl2 concentration by CCK-8 assays,but gradually increased when the concentration increased to 9 mmol · L-1,and the difference approached statistical significance (P ＜ 0.05).Meanwhile,there was significant difference in the viability of the control group (0.592 + 0.055) and cells exposed to 15 mmol · L-1 CaCI2 (0.293 + 0.02) (t =7.811,P ＜0.05).Cell treatment with 15 mmol· L-1 CaC12 for 24 h was the most appropriate condition for HLEC apoptosis,followed by the appearance of nuclear fragmentation and dissolution,enhanced intracellular SOD viability (t =-6.417,P ＜ 0.05),decreased T-GSH content (t =13.816,P ＜ 0.05),and increased ratio of GSSG/T-GSH (t =-4.396,P ＜ 0.05) when compared with the control group,and the differences were statistically significant.Conclusion Intracellular calcium elevation can inhibit the cell viability and increase the levels of SOD and GSSG in HLEC to aggravate the intracellular oxidative damage.关键词
高钙/人晶状体上皮细胞/细胞活力/谷胱甘肽/超氧化物歧化酶Key words
calcium elevation/human lens epithelial cell/cell viability/glutathione/superoxide dismutase分类
医药卫生引用本文复制引用
陈曦,韩子豪,李舒凝,李松蔓,李艳玮,粱皓..高钙状态对人晶状体上皮细胞株SRA01/04氧化应激水平的影响[J].眼科新进展,2017,37(10):906-910,5.基金项目
国家自然科学基金资助(编号:81360146)National Natural Science Foundation of China (No:81360146) （编号:81360146）
