眼科新进展2017，Vol.37Issue(10)：922-925,4.DOI:10.13389/j.cnki.rao.2017.0234
人参皂苷RG3对脉络膜新生血管的抑制作用
Inhibitory effect of ginsenoside RG3 on choroidal neovascularization
摘要
Abstract
Objective To observe the inhibitory effect of ginsenoside RG3 on choroidal neovascularization in vitro and in vivo.Methods MTT assay was used to evaluate the inhibitory effect of ginsenoside RG3 on human umbilical vein endothelial cells (HUVEC) proliferation in vitro,and HUVEC were divided into normal group,in which the cells were cultured in DMEM/F-12 medium containing 10％ fetal bovine serum,control group with its medium containing 2 g · L-1 dimethyl sulfoxide (DMSO) and different concentrations of ginsenoside RG3 administration group (12.5 μmol · L-1,25.0 imol · L-1,50.0 μmol · L-1,100.0 μmol · L-1).Then the absorbance value was measured after 6 h.Then,a small amount of HUVEC was collected again and divided into control group with its medium containing 2 g · L-1 DMSO and 100.0 μrnol · L-1 ginsenoside RG3 group for detecting the inhibitory effect of ginsenoside RG3 on tubular formation and vascular endothelial growth factor (VEGF) protein expression by Western blots.In vivo,20 male C57BL/6J mice were collected and randomly divided into control group and ginsenoside RG3 group.After 2 weeks,followed by establishment of model with a semiconductor laser,fundus fluorescein angiography was performed on the 1 st day and 21 st days after treatment.Results MTT results showed that absorbance value of the normal group,control group,12.5 μnol · L-1,25.0 μmol · L-1,50.0 μmol · L-1,100.0 μmol · L-1 ginsenoside RG3 group was 0.43 +0.17,0.43 ±0.05,0.33 +0.02,0.24 +0.02,0.18 ±0.01,0.15 ±0.01 accordingly,and there was no significant difference between the control group and the normal group (all P ＞ 0.05),but the difference between the other group and control group was statistically significant (all P ＜ 0.05).Tubular formation assay showed that the number of tubular formation in the control group and 100.0 μmol · L-1 ginsenoside RG3 group was 72.5 + 5.56 and 11.33 ± 3.71,respectively,and the difference was statistically significant (P ＜ 0.01).Western blots showed that the relative expression of VEGF in 100.0 μmol · L-1 ginsenoside RG3 group (0.14 ±0.01) was significantly lower than that in the control group (0.46 ±0.01),and the difference was statistically significant (P ＜0.01).In vivo fundus fluorescein anglography showed that the fluorescein leakage area of ginsenoside RG3 group was lower than that of the control group.Conclusion Ginsenosid RG3 can inhibit the formation of choroidal neovascularization by inhibiting the expression of VEGF in vitro and in vivo.关键词
年龄相关性黄斑变性/脉络膜新生血管/人参皂苷RG3/血管内皮生长因子Key words
age-related macular degeneration/choroidal neovascularization/ginsenoside RG3/vascular endothelial growth factor分类
医药卫生引用本文复制引用
卢悦,蔡玲,朱晨,刘格格,张园园,黄建华,刘华..人参皂苷RG3对脉络膜新生血管的抑制作用[J].眼科新进展,2017,37(10):922-925,4.基金项目
辽宁省科学技术基金[面上项目(原优秀人才培育)](编号:201602298) （原优秀人才培育）
辽宁省科技厅-锦州医科大学联合基金项目(编号:20170540382)Liaoning Science and Technology Fund [Project on the Surface (Original Talent Cultivation)](No:201602298) （编号:20170540382）
Liaoning Science and Technology Department Jinzhou Medical University Joint Fund Project (No:20170540382) （No:20170540382）
