原子能科学技术2017，Vol.51Issue(10)：1886-1892,7.DOI:10.7538/yzk.2017.youxian.0014
γ电离辐射对斑马鱼胚胎肝、脾和肾发育形态学的影响
Effect of γ-ray Irradiation on Developmental Morphology of Liver,Spleen and Pronephros in Zebrafish Embryo
摘要
Abstract
Zebrafish was used as a model organism to study the effects of γ-ray irradia-tion on the developmental morphology of important organs in the aquatic organisms. Different doses of gamma rays (0.25,0.5,1 Gy)at the dose rate of 47.79 cGy/min were used to irradiate zebrafish embryos at early development stage (5 hours post-fertili-zation,5 hpf).The changes of yolk sac area at 3 days post-fertilization (3 dpf)and the changes of the morphology of liver,spleen and pronephros at 3 dpf and 5 dpf after γ-ray irradiation were analyzed,respectively.It is found that the area of yolk sac in larval zebrafish at 3 dpf increases with γ-ray irradiation dose in a dose-dependent manner.The vacuoles increase and some cavities occur in liver of larval zebrafish at 3 dpf.The devel-opmental morphology of liver in larval zebrafish at 5 dpf is abnormal,including the reduction in liver size,the increase of vacuoles in liver cells,the deformation of hepato-cyte nucleus,the relatively small hepatic sinusoid and the deformation of nucleated erythrocyte and so on,but the developmental morphology of pronephros and spleen are normal.The ultrastructure of liver cells at 3 dpf shows that γ-ray irradiation can impair mitochondria of liver cells,broaden the intermembrane space of cell nuclei,spoil the arrangement of rough endoplasmic reticulum and so on.The results show that γ-ray irradiation can inhibit the absorption of the yolk in embryos in a dose-dependent manner and change the microscopic and submicroscopic structure of the livers in embryos.关键词
斑马鱼胚胎/γ辐照/形态学/生物标志物Key words
zebrafish embryo/γ-ray irradiation/morphology/biomarker分类
生物科学引用本文复制引用
冯永富,胡南,丁德馨,赵维超,胡淼..γ电离辐射对斑马鱼胚胎肝、脾和肾发育形态学的影响[J].原子能科学技术,2017,51(10):1886-1892,7.基金项目
国防基础重点基金资助项目(B3720132001) （B3720132001）
国家自然科学基金重点项目资助(U1401231) （U1401231）
国防基础科研计划资助项目(JCKY2016403C001) （JCKY2016403C001）
