中国病理生理杂志2017，Vol.33Issue(9)：1598-1601,4.DOI:10.3969/j.issn.1000-4718.2017.09.010
染料木黄酮对氨诱导的星形胶质细胞NF-κB活化的影响
Effect of genistein on ammonia-induced NF-κB activation in astrocytes
摘要
Abstract
AIM:To explore the effect of genistein on ammonia-induced nuclear factor-κB (NF-κB) activation and the underlying mechanism.METHODS:Primary astrocyte cultures were prepared and challenged with NH4Cl to establish a hyperammonemic model.The activation of ERK,Akt and NF-κB was examined by Western blot.RESULTS:AG1478 and genistein significantly inhibited ammonia-induced activation of ERK and Akt.Ammonia-induced NF-κB nuclear translocation was significantly inhibited by the pretreatment of LY294002,genistein and AG1478.CONCLUSION:Genistein significantly inhibited ammonia-induced ERK activation and Akt-mediated NF-κB activation,which might represent the important mechanism by which this naturally occurring substance exerts its swelling-inhibiting effect.关键词
染料木黄酮/氨/星形胶质细胞/核因子κBKey words
Genistein/Ammonia/Astrocytes/Nuclear factor-κB分类
医药卫生引用本文复制引用
杨丹,贾桂枝,楚明会,戴红良..染料木黄酮对氨诱导的星形胶质细胞NF-κB活化的影响[J].中国病理生理杂志,2017,33(9):1598-1601,4.基金项目
辽宁省自然科学基金资助项目(No.2015020360) （No.2015020360）
辽宁医学院校长基金-奥鸿博泽基金(No.XZJJ20140111) （No.XZJJ20140111）
2015年辽宁省大学生创新创业训练计划项目(乙类)(No.201510160000035) （乙类）
辽宁医学院校长基金-奥鸿博泽大学生科技创新基金(No.2015D20) （No.2015D20）
