中国病理生理杂志2017，Vol.33Issue(9)：1611-1618,8.DOI:10.3969/j.issn.1000-4718.2017.09.013
汉防己甲素对人鼻咽癌CNE1和CNE2细胞株的放疗增敏作用
Effect of tetrandrine on radiosensitivity of nasopharyngeal carcinoma cells
摘要
Abstract
AIM:To investigate the mechanism of the radiosensitizing effect of maximum non-cytotoxic doses of tetrandrine (Tet) on nasopharyngeal carcinoma cell lines CNE1 and CNE2.METHODS:The cells were treated with maximum non-cytotoxic doses of Tet (for CNE1 cells at 1.5 μmol/L and for CNE2 cells at 1.8 μmol/L),irradiation at 4 Gy,or combination of irradiation and maximum non-cytotoxic doses of Tet.The cell cycle distribution was analyzed by flow cytometry.The protein levels of γ-H2AX,cleaved caspase-3,p-CDC25C,CDK1,p-CDK1,cyclin B1,ERK and p-ERK were determined by Western blot.RESULTS:The expression of γ-H2AX was increased in CNE1 cells and CNE2 cells after combined treatment with irradiation and maximum non-cytotoxic doses of Tet.The percentages of CNE1 cells and CNE2 cells at G2/M phase in irradiation group were (18.09 ±0.42)％ and (18.48 ± 1.32)％,respectively,which were decreased to (15.88 ± 1.04) ％ and (13.80 ± 0.82) ％ in combined treatment group,respectively (P ＜ 0.05).Combined treatment enhanced the increase in the protein level of cleaved caspase-3 caused by irradiation.The protein levels of pCDC25C and p-CDK1 were increased in a dose-dependent manner by Tet treatment (P ＜ 0.05),while the expression of CDK1 showed no difference among different doses of Tet treatments.The protein levels of p-CDC25C,p-CDK1 and CDK1 showed no difference after the treatment with maximum non-cytotoxic doses of Tet.The combined treatment with irradiation and the maximum non-cytotoxic doses of Tet decreased the protein levels of p-CDC25C and p-CDK1 (P ＜0.05),increased the expression of cyclin B1,and had no influence on the expression of CDK1 （ P ＜0.05).The combined treatment resulted in an increase in the protein level of p-ERK1 (P ＜ 0.05).CONCLUSION:The maximum non-cytotoxic doses of Tet enhance the DNA damage and apoptosis in CNE1 cells and CNE2 cells caused by irradiation,and the mechanism might be associated with ending of G2/M arrest via activation of ERK/CDC25C/CDK1/cyclin B1 pathways.关键词
汉防己甲素/鼻咽癌/G2/M期阻滞/ERK/CDC25C/CDK1/cyclin B1通路Key words
Tetrandrine/Nasopharyngeal carcinoma/G2/M phase arrest/ERK/CDC25C/CDK1/cyclin B1 pathways分类
医药卫生引用本文复制引用
王君,常利红,李霞,陈贤珍,吴喜福,王志远,黄子真,黄健聪,张革化..汉防己甲素对人鼻咽癌CNE1和CNE2细胞株的放疗增敏作用[J].中国病理生理杂志,2017,33(9):1611-1618,8.基金项目
广东省自然科学基金资助项目(No.2014A030313142) （No.2014A030313142）
广州市科技计划对外科技合作专项(No.2013J4500013) （No.2013J4500013）
广州市天河区科技计划项目医疗卫生专项(No.2013KW027) （No.2013KW027）
