中国病理生理杂志2017，Vol.33Issue(10)：1746-1750,5.DOI:10.3969/j.issn.1000-4718.2017.10.003
多巴胺通过mTOR-EAAT2通路影响星形胶质细胞谷氨酸摄取能力
Effect of dopamine on glutamate-uptake ability of astrocytes by regulating mTOR-EAAT2 pathway
摘要
Abstract
AIM:To investigate the effect of dopamine ( DA) on the glutamate ( Glu)-uptake ability of astro-cytes, and the role of mammalian target of rapamycin (mTOR)-excitatory amino acid transporter 2(EAAT2) pathway in this process .METHODS:Extracellular Glu levels in DA-treated primary cortical astrocytes ( PCAs) were measured by a fluorimetric method .The relative expression of EAAT 2 and mTOR at mRNA and protein levels was measured by RT-qPCR and Western blot .PCAs stimulated with or without DA in the presence or absence of mTOR antagonist rapamycin or mTOR agonist MHY1485 were used to determine the expression of mTOR and EAAT 2, and Glu content in the culture supernatant was also measured.RESULTS: The expression of mTOR in DA-treated PCAs was decreased, the expression of EAAT2 was also decreased .Extracellular Glu levels of DA-treated PCAs were elevated significantly .When the PCAs were stimula-ted with DA in the presence of rapamycin , the expression of EAAT2 was decreased , and the levels of extracellular Glu was significantly increased.In the presence of MHY1485, the expression of EAAT2 was elevated, and significant decrease in the levels of extracellular Glu was also observed .CONCLUSION:DA interacts with mTOR-EAAT2 pathway to reduce the Glu-uptake ability of the astrocytes , and causes extracellular Glu accumulation , ultimately destroys the function of astro-cytes.关键词
多巴胺/谷氨酸/星形胶质细胞Key words
Dopamine/Glutamate/Astrocytes分类
医药卫生引用本文复制引用
温芳芳,徐竹,刘乐平,杨建静,丁赛丹..多巴胺通过mTOR-EAAT2通路影响星形胶质细胞谷氨酸摄取能力[J].中国病理生理杂志,2017,33(10):1746-1750,5.基金项目
国家自然科学基金资助项目(No.81671042 （）
No.81300308) （）
