中国病理生理杂志2017，Vol.33Issue(10)：1837-1844,8.DOI:10.3969/j.issn.1000-4718.2017.10.017
RAS突变和微卫星不稳定与Ⅲ期结直肠癌患者临床病理特征及预后的相关性分析
Correlation of RAS mutations and microsatellite instability with clinico-pathological features and prognosis of patients with stage Ⅲ colorectal cancer
摘要
Abstract
AIM:To investigate the status of RAS/BRAF mutations and microsatellite instability ( MSI ) and their associations with clinicopathological features and prognosis of the patients with stage Ⅲ colorectal cancer ( CRC ) . METHODS:The surgical patients with stage ⅢCRC (n=281) were followed up.The mutations of RAS/BRAF were ex-amined by PCR amplification-Sanger sequencing , and MSI status was detected using immunohistochemistry ( IHC) in their archival paraffin-embedded tissue specimens .The relationships of the status with the clinicopathological features and prog-nosis of the patients were statistically analyzed .RESULTS: Among 281 patients, the mutations of RAS/BRAF were ob-served in 136 cases (48.4%), including 116 cases (41.3%) of KRAS mutations.RAS/BRAF mutations were highly cor-related with the level of carcino-embryonic antigen (P<0.05).Moreover, 18 cases (6.4%) of MSI-high (MSI-H) pa-tients were determined by IHC, and MSI-H status was more common in N2b patients (P<0.05).Correlation study found that the mutation rate of BRAF was higher in MSI-H tumors than that in MSI-low ( MSI-L)/microsatellite stability ( MSS) counterparts (P<0.01), although no association between KRAS/NRAS mutations and the MSI status was observed .The prognosis in the patients with wild-type RAS/BRAF or MSI-H was better than the patients with any mutation or MSI-L/MSS (P<0.01).CONCLUSION:Mutant RAS/BRAF and MSI may serve as fairly good indicators for prognosis of the patients with stage Ⅲ CRC.关键词
结直肠癌/RAS基因/BRAF基因/微卫星不稳定Key words
Colorectal cancer/RAS gene/BRAF gene/Microsatellite instability分类
医药卫生引用本文复制引用
刘建化,黄成智,曾卫强,黎莹,杨冬阳,马冬..RAS突变和微卫星不稳定与Ⅲ期结直肠癌患者临床病理特征及预后的相关性分析[J].中国病理生理杂志,2017,33(10):1837-1844,8.基金项目
广东省医学科研基金资助项目(No.A2017418) （No.A2017418）
