中国病理生理杂志2017，Vol.33Issue(10)：1901-1905,5.DOI:10.3969/j.issn.1000-4718.2017.10.028
外源性硫化氢对小鼠原代肝细胞内脂质自噬分解的影响
Effect of exogenous hydrogen sulfide on lipophagy in mouse primary hep-atocytes
摘要
Abstract
AIM:To evaluate the effect of exogenous hydrogen sulfide ( H2 S) from GYY4137 on lipophagy in mouse primary hepatocytes .METHODS:The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion method were divided into 4 groups:the cells in control group were incubated with normal medium; the cells in model group were incubated with 1.2 mmol/L oleic acid (OA) for 48 h;the cells in H2S group or propargylglycine (PAG) group were incubated with 1.2 mmol/L OA for 48 h followed by serum-free phenol red-free RPMI-1640 medium which con-tained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h.The cells were collected to conduct immunofluorescence staining of LC3 and photography under fluorescence microscope , phase-contrast microscope or transmission electron microscope . The protein expression of LC 3-Ⅰ/Ⅱin the hepatocytes was determined by Western blot .RESULTS:In contrast with the model group, the fluorescent particles of LC3, the protein expression of LC3, the number of autophagic lysosome and vacuoles in hepatocytes in H 2 S group increased .CONCLUSION: In steatosis hepatocytes , exogenous H 2 S promotes the lipophagy .关键词
硫化氢/脂解/肝细胞/自噬Key words
Hydrogen sulfide/Lipolysis/Hepatocytes/Autophagy分类
医药卫生引用本文复制引用
王红钢,张优敬,皇甫超申,陈明亮,王军,吴东栋,谢振兴,李彦章,吉爱玲..外源性硫化氢对小鼠原代肝细胞内脂质自噬分解的影响[J].中国病理生理杂志,2017,33(10):1901-1905,5.基金项目
国家自然科学基金资助项目(No.31300884) （No.31300884）
河南省高校重点科研项目(No.16A310001) （No.16A310001）
