中国动脉硬化杂志2017，Vol.25Issue(9)：879-884,6.
亲环素A对THP-1源性泡沫细胞ABCA1表达及胆固醇流出的影响及机制
Effects of cyclophilin A on ABCA1 expression and cholesterol efflux in THP-1 derived macrophages and its mechanism
摘要
Abstract
Aim To investigate the effects and mechanisms of cyclophilin A (CypA) on the expression of ATP binding cassette trasporter A1 (ABCA1) and cholesterol efflux in THP-1 derived macrophages.Methods THP-1 cells were incubated with 160 nmol/L phorbol ester to transform into macrophages.THP-1 derived macrophages were exposed to 50 mg/L oxidized low density lipoprotein (ox-LDL) and cultured with typical approaches.Liquid scintillation counter was used to determine the efficiency of cholesterol efflux.Lipids contents were tested with high performance liquid chromatogram (HPLC).Real-time PCR and Western blot were used to quantify the expression of ABCA1 and nuclear factorκB (NF-κB) nuclear translocation.Parthenolide was used as the specific inhibitor of NF-κB.CD147 was silenced with siRNA.Results CypA significantly reduced cholesterol efflux,promoted NF-κB nuclear translocation and downregulated ABCA1 expression.NF-κB inhibitor parthenolide interfered CypA-prevented ABCA1 expression and cholesterol efflux.After CD147 were silenced with siRNA,CypA inhibited NF-κB nuclear translocation,upregulated ABCA1 expression and promoted cholesterol efflux.Conclusion CypA prevented THP-1 derived macrophages cholesterol efflux by activing NF-κB in a CD147 dependent manner and downregulating ABCA1 expression.关键词
亲环素A/三磷酸腺苷结合盒转运体A1/胆固醇流出/核因子κB/CD147Key words
Cyclophilin A/ATP binding cassette trasporter A1/Cholesterol efflux/Nuclear factor-κB/CD147分类
医药卫生引用本文复制引用
唐小二,唐朝克,张敏,李靓,谢巍,龚朵,夏晓丹,成海鹏,赵真旺,陈凌燕..亲环素A对THP-1源性泡沫细胞ABCA1表达及胆固醇流出的影响及机制[J].中国动脉硬化杂志,2017,25(9):879-884,6.基金项目
国家自然科学基金项目(81570408、81370377和81470569) （81570408、81370377和81470569）
南华大学研究生创新课题(2016XCX04) （2016XCX04）
