中国动脉硬化杂志2017，Vol.25Issue(9)：885-889,5.
D-半乳糖对大鼠肺组织MMP-2表达水平及HA、LN和COL3含量的影响及相关性分析
The D-galactose induced the expression of MMP-2 and the content of HA, LN,COL3 changes and correlation analysis in lung tissue of rats
摘要
Abstract
To investigate the effect of D-galactose on rat pulmonary fibrosis and matrix metalloproteinases (MMP-2) expression and the contents of the hyaluronic acid (HA),Laminin (LN),Collagen type Ⅲ (COL3) in the lung tissue of rat,and to analyze the correlation between MMP-2 and HA,LN,COL3.Methods The SD rats were randomly divided into two groups:normal group and D-galactose group.Morphological changes of lung tissue were observed by HE staining in two groups of rats.The mRNA expression of MMP-2 in the lung tissue in each group was detected by real-time fluorescent quantitative PCR (qRT-PCR).The immunohistochemistry (IHC) and Western blot method were used to detect MMP-2 protein expression in lung tissue.The enzyme-linked immunosorbent assay (ELISA) was used to detect the content of HA,LN and COL3 in rat lung tissue,and the correlation between them was analysed.Results HE staining showed that the lung tissue of D-galactose group had obvious fibrosis changes.The mRNA and protein expression levels of MMP-2 in D-galactose induced group were higher than normal group(P＜0.05),and the contents of COL3 and laminin (LN) were higher than normal rats,but the content of hyaluronic acid (HA) was lower than the normal groups (P＜0.05).Correlation analysis results showed that the expression level of MMP-2 in the rats lung tissue was positively correlated with COL3 and LN,but negatively correlated with HA (P＜0.05).Conclusion D-galactose can cause lung fibrosis changes in aged rats,and could increase the expression level of MMP-2 and the content of COL3 and LN,and decrease the content of HA in the rats lung tissue,and there is a correlation between MMP-2 and COL3,LN,and HA which may provide other diagnostic basis for the detection of pulmonary fibrosis.关键词
D-半乳糖/基质金属蛋白酶2/透明质酸/层粘连蛋白/Ⅲ型胶原Key words
D-galactose/Matrix metalloproteinase-2/Hyaluronic acid/Laminin/Collagen type Ⅲ分类
医药卫生引用本文复制引用
杨松昊,朱光荣,丁宁,杨安宁,蒋袁絮,田珏,姜怡邓,杨晓玲..D-半乳糖对大鼠肺组织MMP-2表达水平及HA、LN和COL3含量的影响及相关性分析[J].中国动脉硬化杂志,2017,25(9):885-889,5.基金项目
国家自然科学基金资助项目(81560242,81360063) （81560242,81360063）
宁夏教育厅项目(NGY2015092) （NGY2015092）
