中国动脉硬化杂志2017，Vol.25Issue(9)：890-894,5.
miR-146b对人单核细胞株THP-1细胞COX2表达的影响及可能机制
Lentiviral-mediated microRNA-146b regulates COX2 expression by p38MAPK
摘要
Abstract
Aim To investigate the effect of microRNA-146b (miR-146b) on the expression of p38 Mitogen-Activated protein kinase(p38MAPK) in human acute monocytic leukemia cell line(THP-1).Methods Cell model was established by angiotensin Ⅱ (Ang Ⅱ) stimulation.Pre-miR-146b-3p or anti-miR-146b-3p were transfected by lentivirus and the expression of p38MAPK and COX2 were detected.p38MAPK siRNA was transfected and the expression of p38MAPK and cyclooxygenase(COX2) in THP-1 cells were detected.Results The expression of p38MAPK and COX2 was increased in THP-1 cells stimulated by Ang Ⅱ and pre-miR-146b-3p could amplify the effect.p38MAPK siRNA decreased the ability of pre-miR-146b-3p to increase levels p38MAPK and COX2.The difference was statistically significant (P＜0.01).Conclusion miR-146b may regulate COX2 expression by p38MAPK in Ang Ⅱ stimulated THP-1 cells,and may play an important role in the diagnosis and treatment of cardiovascular diseases.关键词
miR-146b/人单核细胞株1/p38分裂素原活化蛋白激酶/环氧化酶2/慢病毒Key words
miR-146b/THP-1/p38MAPK/COX2/Lentivirus分类
医药卫生引用本文复制引用
杨铭,陆林,吴方..miR-146b对人单核细胞株THP-1细胞COX2表达的影响及可能机制[J].中国动脉硬化杂志,2017,25(9):890-894,5.基金项目
上海市自然科学基金(16ZR1430900) （16ZR1430900）
