中国动脉硬化杂志2017，Vol.25Issue(9)：895-898,4.
芍药苷对脂多糖诱导的THP-1细胞炎症因子分泌和ABCA1表达的影响
Effect of paeoniflorin on secretion of inflammatory factors and expression of ATP-binding cassette transporter A1 in THP-1 cells
摘要
Abstract
Aim To investigate the effect of paeoniflorin (PF) on secretion of inflammatory factors and expression of ATP-binding cassette transporter A1 (ABCA1) in THP-1 cells.Methods After pretreatment with PF (10-8,10-7,10-6,10-5 and 10-4 mol/L) for 0.5 h,THP-1 cells was co-treated by LPS (1 mg/L) and PF for 24 h.Levels of interleukin-1β (IL-1β),interleukin-6 (IL-6),interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in supernatant of cell culture medium were tested by enzyme linked immunosorbent assay (ELISA).Expression of ABCA1 in THP-1 cells were measured by Western blot.Results Compared with control group,the levels of IL-1β,IL-6,IL-8 and TNF-α in supernatant of cell culture medium were significantly increased (all P＜0.05) and expression of ABCA1 was significantly down-regulated in LPS group (P＜0.05).Compared with LPS group,the levels of IL-1β,IL-6,IL-8 and TNFα in supernatant of cell culture medium were significantly decreased (all P＜0.05) and expression of ABCA1 was significantly up-regulated in LPS+PF (10-6,10-5 and 10-4 mol/L) group (all P＜0.05).Conclusion Paeoniflorin inhibits the secretion of inflammatory factors and down-regultion of ABCA1 expression induced by LPS in THP-1 cells.关键词
芍药苷/脂多糖/炎症因子/三磷酸腺苷结合盒转运体A1Key words
Paeoniflorin/Lipopolysaccharide/Inflammatory factors/ATP-binding cassette transporter A1分类
医药卫生引用本文复制引用
袁娜,朱明燕,杨宏发,曾高峰,王波..芍药苷对脂多糖诱导的THP-1细胞炎症因子分泌和ABCA1表达的影响[J].中国动脉硬化杂志,2017,25(9):895-898,4.基金项目
衡阳市科技计划项目(2015KJ58) （2015KJ58）
