中国农业科技导报2017，Vol.19Issue(10)：21-27,7.DOI:10.13304/j.nykjdb.2016.657
陆地棉钾吸收相关基因GhHAK1的克隆与功能分析
Cloning and Fuctional Characterization of a Potassium Absorption Related Gene GhHAK1 from Cotton (Gossypium hirsutum L.)
摘要
Abstract
Cotton is an important economic crop typically with preference for potassium.Its yield and quality are closely related to potassium content.In this study,open read frame of GhHAK1 was cloned from the upland cotton.The dendrograms of GhHAK1 showed that it belonged to the Ⅰ subtribe,which was considered to be an important component of high-affinity system for K+.The trans-membrane region prediction reveals that the protein coded by this gene with 12 trans-membrane domains (TMS),and it had a long cytoplasmic ring between the second and third TMS.The sub-cellular localization experiment of onion epidermis showed that GhHAK1 was located on plasma membrane.And under the condition of low potassium (0.05 mmol/L) condition,the wild-type Arabidopsis showed obvious symptoms of K deficiency,the leaves turned yellow and bolting was obviously inhibited.But the transgenic plants' growth was not affected.Besides,the content of K+ in transgenic Arabidopsis leaves was double that of the wild type and the content of K+ in root was about 1.5 times.The results showed that GhHAK1 might possess efficient potassium absorption function and would provide important genetic resources for further breeding new cotton varieties adapted to ootassium deficiency soil and saline environment.关键词
陆地棉/GhHAK1/克隆/低钾胁迫Key words
cotton (Gossypium hirsutum L.)/GhHAK1/clone/low potassium stress分类
生物科学引用本文复制引用
王昭玉,刘建凤,吕婷婷,李爱芹,王頔,张子玉,闫瑞娴,陈佳雪,董惠心,唐宁蔓..陆地棉钾吸收相关基因GhHAK1的克隆与功能分析[J].中国农业科技导报,2017,19(10):21-27,7.基金项目
河北省自然科学基金项目(C2015201227) （C2015201227）
河北大学人才引进项目(2014-277)资助. （2014-277）
