沙门菌tcpS基因的血清型分布及其在特定血清型鉴定中的应用
Serotype distribution of Salmonella tcpS gene and its application in identification of specific serovars
摘要
Abstract
A tcpS-based PCR method was established to simultaneously screen Salmonella enterica serovars Enteritidis,Pullorum/Gallinarum,and Dublin.The developed PCR method provides laboratorial support as a convenient and rapid approach for epidemiological investigation,and tcpS can be a potential candidate gene for the development of PCR-based Salmonella identification.The serotype distribution of Salmonella tcpS gene was analyzed by bioinformatic approach.The specificity and sensitivity of the PCR method were determined based on 27 different Salmonella serovars and 10 non-Salmonella strains.The PCR method was applied to clinical Salmonella isolates from one pig farm (48 isolates),one chicken farm (22 isolates) and one cattle farm (11 isolates) from Jiangsu Province.In silico analysis showed that tcpS existed only in Salmonella Enteritidis,Pullorum/Gallinarum,and Dublin.The developed PCR method had potent specificity and sensitivity,and could screen the three specific Salmonella serovars accurately.The coincidence rate of the clinical sample detection was up to 100％.The tcpS-based PCR detection method could screen Salmonella Enteritidis,Pullorum/Gallinarum,and Dublin accurately,and could be an assistant method to the traditional serotyping method.Furthermore,the novel tcpS gene can be a potent gene candidate for the development of PCR method for the identification of Salmonella serovars.关键词
沙门菌/tcpS基因/PCR/血清型Key words
Salmonella/tcpS gene/PCR/serotype分类
医药卫生引用本文复制引用
熊丹,宋丽,陶静,郑慧娟,周子皓,潘志明,焦新安..沙门菌tcpS基因的血清型分布及其在特定血清型鉴定中的应用[J].中国人兽共患病学报,2017,33(9):757-762,6.基金项目
国家重点研发计划课题(2016YFD0501607) （2016YFD0501607）
公益性行业(农业)科研专项(201403054) （农业）
国家自然科学基金(31320103907,31230070) （31320103907,31230070）
江苏省重点研发项目(BE2015343) （BE2015343）
江苏省第9批“六大人才高峰”高层次人才项目(NY-028) （NY-028）
扬州大学科技创新团队 （）
江苏省高校优势学科建设工程项目联合资助 Funded by the National Key Research and Development Program Special Project (No.2016YFD0501607),the Special Fund for Agroscientific Research in the Public Interest (No.201403054),the National Natural Science Foundation of China (Nos.31320103907 and 31230070),the Research and Development Program of Jiangsu (No.BE2015343),the "Six Talent Peaks Program" of Jiangsu Province (No.NY-028),the Yangzhou University Science and Technology Innovation Team,and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) （No.2016YFD0501607）
