中国人兽共患病学报2017，Vol.33Issue(9)：774-778,5.DOI:10.3969/j.issn.1002-2694.2017.09.004
弓形虫微线蛋白16抗原表位区的预测及酵母表面展示
Prediction and yeast surface display of antigenic epitope region of TgMIC16 of Toxoplasma gondii
摘要
Abstract
The bioinformatics software was used to predict the B cell and T cell epitopes of Toxoplasma gondii microneme 16 (TgMIC16) followed by epitopes display on the yeast of Saccharomyces cerevisiae.B and T cell epitopes of TgMIC16 were predicted by DNAStar and IEDB,and software of SYFPEITHI and BIMAS,respectively.According to the predicted results,the antigenic epitope region was expressed with pCTCON2/EBY100 display system.The expression protein was detected by indirect immunofluorescence (IFA) and flow cytometry.Results showed that there were potential B/T cell epitopes in TgMIC16 and concentrated in the aa343-625 region.The epitope region was successfully displayed on the surface of yeast cells,and the optimal induction time was 24 hours.The study would lay the foundation for the development and efficacy evaluation of the yeast carrier vaccine in the further research.关键词
酵母细胞表面展示/抗原表位/微线蛋白-16Key words
yeast surface display/antigen epitope/microneme protein-16分类
医药卫生引用本文复制引用
孙慧,李瑾,肖婷,徐超,尹昆,黄炳成,雷战,魏庆宽..弓形虫微线蛋白16抗原表位区的预测及酵母表面展示[J].中国人兽共患病学报,2017,33(9):774-778,5.基金项目
国家自然科学基金(No.81501770) （No.81501770）
山东省自然科学基金(No.ZR2015YL051,No.ZR2015YL033,No ZR2016CP18) （No.ZR2015YL051,No.ZR2015YL033,No ZR2016CP18）
山东省医学科学院医药卫生科技创新工程 Supported by the National Natural Science Foundation of China (No.81501770),the Shandong Natural Science Foundation (Nos.ZR2015YL051,ZR2015YL033 and ZR2016CP18),and the Innovation Project of Shandong Academy of Medical Sciences （No.81501770）
