中国人兽共患病学报2017，Vol.33Issue(9)：779-783,5.DOI:10.3969/j.issn.1002-2694.2017.09.005
抗博尔纳病病毒磷蛋白单克隆抗体的制备及鉴定
Preparation and identification of monoclonal antibody against phosphoprotein of Borna disease virus
摘要
Abstract
In order to prepare the monoclonal antibodies (MAbs) by the recombinant phosphoprotein (p24) of Borne dis ease virus (BDV) as immunogen,the spleen cells of immunized balb/c mice with the recombinant BDV p24 were fused with the myeloma cells SP2/0.The hybridoma cell lines secreting MAbs against p24 were obtained after selected by indirect ELISA and subcloned for 3 times.The MAbs were prepared by intraperitoneal injection in mice,purified by affinity chromatography,identified by western blot and immunofluorescence assay (IFA) for specificity.The purified MAbs against BDV p24 from ascites belonged to IgG1 showed a purity of 98％ and 93％,and a titer of 1 ∶ 81 000,and specific reaction with the BDV p24 restructured and expressed in Oligodendroglia cells (OL).The MAbs against BDV p24,with high specificity and sensitivity,were prepared successfully,which laid a basis for the study of diagnostic reagents and pathogenic mechanism of BDV.关键词
博尔纳病病毒/磷蛋白类/单克隆抗体Key words
Borna disease virus (BDV)/phosphoproteins/monoclonal antibody分类
医药卫生引用本文复制引用
马丽华,邓榕,徐晓艳,杨宏静,谢鹏..抗博尔纳病病毒磷蛋白单克隆抗体的制备及鉴定[J].中国人兽共患病学报,2017,33(9):779-783,5.基金项目
国家重点基础研究发展计划(973计划,No.2009CB918300)Supported by the National Basic Research Program of China (973 Program,Grant No.2009CB918300) （973计划,No.2009CB918300）
