中国人兽共患病学报2017，Vol.33Issue(9)：784-788,5.DOI:10.3969/j.issn.1002-2694.2017.09.006
四川省嗜肺军团菌血清Ⅰ型(LP1)基因分型研究
Gene typing of Legionella pneumophila serogroup 1 in Sichuan Province, China
摘要
Abstract
The aim of the research is to investigate the genetic characteristics of Legionella pneumophila serogroup1 (LP1)in Sichuan Province.The sequence-based typing (SBT) and multiple-locus VNTR analysis (MLVA) were used to describe the genetic polymorphism of 42 strains which were isolated from 1989-2016 in Sichuan Province,China.According to the reference,PCR was used to detect the 8-VNTR loci and 7 housekeeping genes respectively.The VNTR results were determined by using capillary electrophoresis,and the SBT results were sequenced and compared with the database of EWGLI.Results showed that totally 42 stains were divided into 8 MLVA types with the advantage types were M08 (47.6 ％) and M07 (23.8 ％).Twelve ST types were obtained with 3 main clonal complex and 2 singleton,including 2 novel ST types,among those,ST1 was the predominant type,accounting for 52.3 ％,following by ST630 (14.2 ％).In conclusion,our results demonstrated MLVA and SBT were both applied to the research for molecular epidemiological investigation of LP1 and showed the high genetic polymorphism and the regional specificity.The results also suggest that the isolates are a potential threat to the public,effective control and prevention strategies are urgently needed.关键词
四川省/嗜肺军团菌血清Ⅰ型/基因分型Key words
Sichuan Province/Legionella pneumophila serogroup1/gene typing分类
医药卫生引用本文复制引用
曾林子,廖虹瑜,祁腾,刘谊,刘莉莉,罗隆泽..四川省嗜肺军团菌血清Ⅰ型(LP1)基因分型研究[J].中国人兽共患病学报,2017,33(9):784-788,5.基金项目
四川省医学会青年创新课题(No.Q15081)Supported by the Youth Innovation Project of Sichuan Medical Association (No.Q15081) （No.Q15081）
