中国人兽共患病学报2017，Vol.33Issue(9)：793-799,7.DOI:10.3969/j.issn.1002-2694.2017.09.008
山东省动物源沙门氏菌MLST和血清分型与分布研究
Distribution and typing of animal-derived Salmonella with MLST and serotype
摘要
Abstract
To compare the discriminatory ability of multilocus sequence typing and serotype of animal-derived Salmonella and find its distribution in Shandong Province,78 chicken-origin,56 duck-origin and 20 swine-origin Salmonella were separated from some regions of Shandong Province.Seven conserve sequences of Salmonella were PCR-amplified for MLST and slide agglutination test for serotyping.Results showed that by serotyping,6 serotypes were identified from chicken-origin Salmonella,including 88.5％0 S.enteritidis,5.1％ S.indiana,2.6％ S.thompson,1.3％ S.typhimurium,1.3％ S.senftenberg,1.3％ S.agama.Two serotypes were identified from duck-origin Salmonella,including 67.9％ S.enteritidis,32.1％ S.ty phimurium.Three serotypes were identified from swine-origin Salmonella,including 65％ S.typhimurium,20％ S.derby,and 15％ S.enteritidis.By MLST typing,seven ST types were identified from chicken-origin Salmonella:ST11,ST19,ST26,ST128,ST14,ST17 and Newl.Three ST types were identified form duck-origin Salmonella:ST11,ST19 and New2.Three ST types were identified from swine-origin Salmonella:ST34,ST40 and ST3007.Overall,the types identified with two methods were closed,so MLST and serotype have similar discriminatory ability.关键词
沙门氏菌/血清型/MLST/分型Key words
Salmonella/serotype/MLST/typing分类
医药卫生引用本文复制引用
赵翠,张庆,郭树源,李璐璐,刘玉洁,王永明,常维山,刘玉庆..山东省动物源沙门氏菌MLST和血清分型与分布研究[J].中国人兽共患病学报,2017,33(9):793-799,7.基金项目
山东省农业科学院科技创新工程(CXGC2016A10),山东省农业重大应用技术创新项目(SDAIT-11-09)Supported by the Shandong Provincial Major Projects of Independent Innovation,Shandong Dhicken Public Health Model Establishment and Demonstration,Shandong Academy of Agricultural Science and Technology Innovation Project (No.CXGC2016A10) （CXGC2016A10）
