中国人兽共患病学报2017，Vol.33Issue(9)：814-820,7.DOI:10.3969/j.issn.1002-2694.2017.09.012
沙雷菌裂解性噬菌体PS2生物学特性及其全基因组测序
Characterization and genomic sequencing of PS2, a lytic phage that infects Serratia marcescens clinical isolates
摘要
Abstract
The present study focused on the characterization and genomic sequence of phage PS2 that infects Serratia marcescens clinical isolates.The morphology of phage PS2 was observed with electron microscope.The one-step growth curve,host range,and stability of PS2 were investigated.In addition,Phage DNA was extracted from the purified phage particles using a MiniBEST Viral RNA/DNA Extraction Kit.DNA sample was analyzed by digesting with restriction enzymes.The phage DNA was used for constructing the sequencing library.The library was sequenced on a MiSeqTM platform.The whole genome sequence was obtained by Velvet (version:1.2.08) assembling.Phage PS2 belongs to the Myoviridae family.The linear,circularly permuted,167 266-bp double-stranded DNA genome of PS2 has high similarities to T4-1ike phages.The phage DNA contains 41.7％ GC and 276 ORFs.PS2 exhibited a 21-minute latent period and 70 PFU per cell at burst size when the pathogenic S.marcescens strain S2 served as a host.Further investigation suggested that PS2 is stable in a wide pH range (pH5 to pH10) and at extreme temperatures (50 ℃ and 60 ℃) after incubation alone at different pHs and different temperatures,respectively.The paper focused on the isolation and identification of a novel lytic S.marcescens phage,the biological characteristics,the whole genome sequencing and the preliminary study of bioinformatics,which laid the foundation for deeply analysis to the phage therapy of multi-drug resistant bacteria and the phage biological information.关键词
粘质沙雷菌/噬菌体/生物学特性/基因组测序Key words
Serratia marcescenes/phage/characterization/genomic sequencing分类
生物科学引用本文复制引用
张改,王中全,李振江,靳静,王书伟,陈松建,李亚辉,黄德海,王小亭,王山梅..沙雷菌裂解性噬菌体PS2生物学特性及其全基因组测序[J].中国人兽共患病学报,2017,33(9):814-820,7.基金项目
河南省科技厅重点科技攻关项目(No.142102310440),河南省医学科技攻关计划项目(No.201303199)Supported by the Key Projects of Science and Technology Department of Henan Provincial Science and Technology Department (No.142102310440) and the Project of Medical Science and Technology Project of Henan Province (No.201303199) （No.142102310440）
