中国水稻科学2017，Vol.31Issue(5)：457-464,8.DOI:10.16819/j.1001-7216.2017.7031
水稻硝酸盐转运蛋白基因OsNPF7.9在氮素积累和转运中的功能研究
Function Analyses of Rice Nitrate Transporter Gene OsNPF7.9 in Nitrogen Accumulation and Transport
摘要
Abstract
[Objective] NPF family in plants can transport nitrate and peptide, etc. Functional analyses of OsNPF7.9 can lay a theoretical basis for studying molecular mechanism of high nitrogen use-efficiency.[Method] Bioinformatics of OsNPF7.9 was predicted with different biological softwares. The OsNPF7.9 promoter-GUS transgenic rice was obtained for analyzing the spatial expression pattern of OsNPF7.9. And the expression patterns of OsNPF7.9 under different N supply levels were observed using semi-quantitative RT-PCR and pOsNPF7.9::GUS transgenic rice.OsNPF7.9 overexpressing rice was obtained, and their different physiological indexes were measured.[Result] OsNPF7.9 is localized on plasma membrane with 12 transmembrane domains and a big hydrophilic loop between the 6th and the 7th transmembrane domain. Tissue expression pattern showed that OsNPF7.9 was expressed in roots, leaves, root-shoot junction and flowers. Both RT-PCR and GUS staining of pOsNPF7.9::GUS transgenic rice indicated that the expression of OsNPF7.9 was not affected by N concentration and form. Overexpression of OsNPF7.9 could significantly increase not only nitrate concentration in shoots and roots-shoots nitrate ratio; but also total nitrogen concentration and accumulation in shoots, and roots-shoots total nitrogen ratio.[Conclusion] OsNPF7.9 participated nitrate transport from roots to shoots, and overexpression of OsNPF7.9 could increase N accumulation and transport from roots to shoots.关键词
水稻/氮素/OsNPF7.9/硝酸盐转运蛋白Key words
rice/nitrogen/OsNPF7.9/nitrate transporter分类
生物科学引用本文复制引用
冯慧敏,陆宏,王汉卿,李昕玥..水稻硝酸盐转运蛋白基因OsNPF7.9在氮素积累和转运中的功能研究[J].中国水稻科学,2017,31(5):457-464,8.基金项目
国家自然科学基金资助项目(31401938) （31401938）
中央高校基本科研业务费专项资金资助项目(Y0201500014,Y0201600366). （Y0201500014,Y0201600366）
