中国水稻科学2017，Vol.31Issue(5)：483-488,6.DOI:10.16819/j.1001-7216.2017.7010
一个水稻小热休克蛋白的异源表达及寡聚特性分析
Heterologous Expression and Oligomeric Identification of a Small Heat Shock Protein (SHSP) from Oryza sativa
摘要
Abstract
[Objective] In our previous study, a small heat shock protein gene OsSHSP17.6 was cloned from Oryza sativa and its expression was shown to be significantly up-regulated by heat shock or viral infection, suggesting that the OsSHSP17.6 could play an important role in both biotic and abiotic stress responses. In this study, our objective is to further identify the characteristics of OsSHSP17.6.[Method] The OsSHSP17.6 gene was sub-cloned into the plasmid pET-32a, a prokaryotic expression vector, and transformed into Escherichia coli BL21(DE3)pLysS for inducible expression. Then the recombinant protein was purified with affinity chromatography and used for native PAGE and Western-blotting assays.[Result] Its heterologous expression appeared to alleviate the poisonous effect of IPTG on the hostE.coli. Native PAGE and Western-blotting assays showed that the purified recombinant OsSHSP17.6 could form homological dimers and oligomerin vitro.[Conclusion] Taken together, these findings supported the hypothesis that the protein should be functional molecular chaperonein vivoand indicated that OsSHPS17.6could be involved in the stress response by its homological oligomerization, which could contribute to the functional identification of OsSHSP17.6.关键词
小热休克蛋白/异源表达/寡聚化作用Key words
small heat shock protein (SHSP)/heterologous expression/oligomerization分类
生物科学引用本文复制引用
蔡年俊,郭留明,李静,项聪英,羊健,陈剑平,张恒木..一个水稻小热休克蛋白的异源表达及寡聚特性分析[J].中国水稻科学,2017,31(5):483-488,6.基金项目
国家自然科学基金资助项目(31601603) （31601603）
浙江省自然科学基金资助项目(LQ14C140003). （LQ14C140003）
