中国水稻科学2017，Vol.31Issue(5)：489-499,11.DOI:10.16819/j.1001-7216.2017.7043
水稻淡绿叶基因PGL11的鉴定与精细定位
Identification and Fine Mapping of Pale Green Leaf PGL11 in Rice
摘要
Abstract
[Objective]Leaf is the main site of photosynthesis in rice. The change of leaf color is directly related to the growth and development of rice. The research of leaf color mutants is an important genetic basis for the study of rice functional genomics. [Method]We identified a pale green leaf mutant termed pgl11(pale green leaf 11) from japonica cultivar Nipponbare by ethyl methylsulfonate (EMS) treatment. The chlorophyll content of wild type(WT) and mutant was measured at different growth stages. At the seedling stage, the chloroplast structure of the leaves of the WT and the mutant were observed with a transmission electron microscopy. At the tillering stage, the photosynthetic parameters of WT and pgl11 were measured and the stomatal structure was observed. At the mature stage, the main agronomic traits of WT and pgl11 were determined. F2 population derived from pgl11/Nanjing 6 was used to map this gene by position cloning approach.[Result]At seedling stage, every new leaf of the mutant pgl11 was pale green. As the leaves matured, the leaf color gradually turned green. Compared with wild type, the chlorophyll content in pgl11 decreased at seedling stage. However, there was no significant difference at heading stage. In addition, the photosynthetic rate and stomatal conductance of pgl11 were significantly decreased, but the intercellular CO2 concentration was apparently increased. The stomata was abnormal in pgl11. The agronomic traits including plant height, flag leaf width, secondary rachis branch number, grain number per panicle, grain length, grain width, 1000-grain weight and seed setting rate decreased significantly in pgl11 compared with wild type. The expression of genes associated with chloroplast transcription and translation were upregulated in pgl11, while the expression of chlorophyll synthesis and photosynthesis related genes were downregulated. Genetic analysis showed that the mutant phenotype was controlled by a single recessive nuclear gene, and PGL11 was mapped to a 110kb region between the markers C6 and C8 on the short arm of chromosome 1. [Conclusion]It was suggested that PGL11 gene would be a putative novel pale green leaf gene.关键词
水稻/淡绿叶/叶绿体/遗传分析/基因定位Key words
rice/pale green leaf/chloroplast/genetic analysis/gene mapping.分类
生物科学引用本文复制引用
涂政军,胡江,任德勇,高振宇,董国军,陈光,郭龙彪,钱前,曾大力,邹国兴,黄李超,陈龙,代丽萍,高易宏,冷语佳,朱丽,张光恒..水稻淡绿叶基因PGL11的鉴定与精细定位[J].中国水稻科学,2017,31(5):489-499,11.基金项目
中央级公益性科研院所专项 （）
中国农科院科技创新工程资助项目 （）
国家自然科学基金资助项目(31661143006,91435105). （31661143006,91435105）
