中国水稻科学2017，Vol.31Issue(5)：533-541,9.DOI:10.16819/j.1001-7216.2017.6171
褐飞虱热激蛋白70在不同温度胁迫下的差异表达特性研究
Induced Expression Profiles of Hsp70s in Brown Planthoppers,Nilaparvata lugens, Under Different Temperatures
摘要
Abstract
[Objective]The brown planthopper (BPH),Nilaparvata lugens, is one of the major insect pests on rice in China. In recent years, the overwintering area of BPH expanded with global warming. In order to explore the response mechanisms of BPH to temperature, thehsp70s were cloned and the induced expression profiles of these genes were analyzed.[Method] The nucleotide sequences ofhsp70s were gotten from transcriptome sequencing and GenBank database, the phylogenetic tree of HSP70 was based on neighbor-joining method by MEGA4.1, and the induced expression profiles were analyzed by real-time quantitative PCR.[Result] It was found that fifteen hsp70 homologous genes with complete open reading frame were gotten in BPH, and 8 out of 15 genes were expressed in normal conditions or temperature stress. These eighthsp70s were distributed in the cytoplasm/nucleus, endoplasmic reticulum or mitochondria, and had significantly different expression in normal conditions. After 30-44°Chigh temperature treatment, the expression levels of KX976471, KX976473, KX976475, KX976476, KX976477 and KX976478 were up-regulated, and the maximum increased times was 1.72-245.33. However, only the expression level of KX976475 increased by 2.38 times, the other seven were not changed or even significantly decreased in expression level after 0-22°C temperature treatment.[Conclusion]It was found that eight hsp70s were expressed in normal conditions or temperature stress in the cytoplasm/nucleus, endoplasmic reticulum or mitochondria. Thesehsp70s might play an important role in high temperature adaptation, but a limited role in cold adaptation in BPH.关键词
褐飞虱/温度/热激蛋白70/诱导表达Key words
Nilaparvata lugens/temperature/heat shock protein 70/induced expression分类
生物科学引用本文复制引用
单丹,王利华,张月亮,韩阳春,牛洪涛,潘磊,方继朝..褐飞虱热激蛋白70在不同温度胁迫下的差异表达特性研究[J].中国水稻科学,2017,31(5):533-541,9.基金项目
国家自然科学基金资助项目(31572004 （）
31301660) （）
江苏省自然科学基金资助项目(BK20130711),江苏省农业自主创新资金资助项目(6111608). （BK20130711）
