中国兽医科学2017，Vol.47Issue(9)：1073-1079,7.DOI:10.16656/j.issn.1673-4696.2017.09.001
表达IBDV AH1株VP2嵌合法氏囊素三肽基因的重组火鸡疱疹病毒的构建及免疫功能分析
Construction of recombinant turkey herpesvirus expressing the VP2 protein of infectious bursal disease virus AH1 strain and its immunologic functions
摘要
Abstract
Construction strategy and immune functions of recombinant herpesvirus of infectious bursal disease(IBD) were studied.Non essential region UL44-UL45 of the HVT FC126 genome was selected as insertion sites.Using the selection marker Eco-gpt(Xanthine-guanine phosphoribosyl transferase(gpt),plasmid pUAB-gpt was constructed.Then,BS10-VP2 expression cassette was constructed and cloned into the plasmid pUAB-gpt to obtain the transfer vector of turkey herpesvirus pUAB-Pec-BS10-VP2-Egpt.Finally,transfer vector and total DNAof HVT infected cells were cotransfected into chicken embryo fibroblasts(CEFs).The recombinant rHVT-Pec-BS10-VP2 expressing the VP2 gene of IBDV was identified by indirect immunofluorescence assay(IFA),IBDV sandwichELISAandWestern-blot.Inaddition,the rHVT-Pec-BS10-VP2 were used to immunize 1-day-old SPF chicken.Antibody titrations were detected on 14 day after immunization.Splenic lymphocyte proliferation activity was remarkable on 21 day after immunization.These results demonstrate that the recombinant HVT expressing the VP2 protein of infectious bursal disease virus is proved successful.关键词
传染性法氏囊病病毒/VP2基因/法氏囊素三肽/重组火鸡疱疹病毒Key words
infectious bursal disease virus/VP2 gene/BS10/recombinant rHVT分类
农业科技引用本文复制引用
潘群兴,毕振威,王永山,欧阳伟,王晓丽,孟凯,夏兴霞,诸玉梅..表达IBDV AH1株VP2嵌合法氏囊素三肽基因的重组火鸡疱疹病毒的构建及免疫功能分析[J].中国兽医科学,2017,47(9):1073-1079,7.基金项目
国家重点研发计划项目(2016YFD0500800) （2016YFD0500800）
国家自然科学基金项目(31272537,31302070,31572504) （31272537,31302070,31572504）
江苏省自然科学基金项目(BK20141381,BK20151366) （BK20141381,BK20151366）
江苏省农业科技自主创新资金项目[CX(16)1052] （16）
