中国兽医科学2017，Vol.47Issue(9)：1094-1099,6.DOI:10.16656/j.issn.1673-4696.2017.09.004
鸭瘟病毒在鸭胚脐带间充质干细胞上生物学特性的研究
Biological characteristics of duck plague virus on duck embryo umbilical cord mesenchymal stem cells
摘要
Abstract
In order to understand the adaptability and proliferation of duck plague virus(DPV) in duck embryonic umbilical cord mesenchymal stem cells,DPV virus's biological characteristics in the duck embryo umbilical cord mesenchymal stem cells after the continuous passage for 10 generations was analyzed by observation of CPE and electron microscopy,nucleic acid detection,indirect immunofluorescence detection,and determination of TCID50 of the continuous passage of 10 generations.Lesions were seen by the results of CPE duck plague virus in the duck embryo umbilical cord mesenchymal stem cells on the first generation,and they were stable even after several generations.The progeny virus could be seen under electron microscopy.The fragment of UL6 gene was amplified with a size of 416 bp.It was further confirmed that the positive serum of against DPV could be specific fluorescent staining for infected lesions in indirect immunofluorescence analysis.The virus titer of the continuous 10 generations was not less than 10-6.43.The titer(TCID50) which was determined by DPV on DEUCMSC was significantly higher than that of DEF.It was proved that DPV could proliferate on duck embryonic umbilical cord mesenchymal stem cells.关键词
鸭瘟病毒/鸭胚脐带间充质干细胞/生物学特性Key words
duck plague virus/duck embryo umbilical cord mesenchymal stem cells/biological characteristics分类
农业科技引用本文复制引用
徐丽晶,陆涛峰,王怡平,陈洪岩,赵丽丽..鸭瘟病毒在鸭胚脐带间充质干细胞上生物学特性的研究[J].中国兽医科学,2017,47(9):1094-1099,6.基金项目
国家科技支撑计划项目(2015BAI07B02-02) （2015BAI07B02-02）
中国农业科学院基本科研业务费专项(Y2016PT41) （Y2016PT41）
黑龙江省自然科学基金重点项目(ZD2016006) （ZD2016006）
中央级公益性科研院所基本科研业务费专项(1610302016013,1610302017013) （1610302016013,1610302017013）
