中国兽医科学2017，Vol.47Issue(9)：1106-1111,6.DOI:10.16656/j.issn.1673-4696.2017.09.006
天祝白牦牛前后盘吸虫组织结构的观察
Observation of the organizational structures of the paramphistomum from white yaks in Tianzhu county
摘要
Abstract
To observe the organizational structures of the Paramphistomum from Tianzhu county of Gansu province,paraffin sections were prepared,stained by HE and PAS,and then observed under optical microscopy.In result,the Paramphistomum integumentum were divided into two layers:outer keratinized epithelium and intimal intensive circular muscle.The main part in the body cavity was reproductive system,digestive system and some muscle bundle and fibrous tissues.The reproductive system contained ovaries,uterus and testes.The digestive system spread over the whole body cavity.The sucker was divid ed into three layers:inner,middle,and outer layers.The middle layer contains more radially arranged cells.The outer layer was relatively thin circular muscle.The inner layer was thicker neatly arranged circular and keratinized skin structure.The middle layer of the sucker accounted for a large proportion,which mainly contained radially arranged muscle fibers.The inner part of middle layer contained a large number of basophilus cell,which distributed between the muscle fibers in cluster.Oral sucker was smaller than ventral sucker,and its proportion was about 1∶1.8.PAS staining revealed that glycogen mainly accumulated in reproductive system,and a few in the muscle layer of acetabulum.The squash technique allows us better observation of relative positions of various structures.关键词
白牦牛/前后盘吸虫/石蜡切片/HE染色/糖原染色Key words
white yak/Paramphistomum/paraffin section/HE staining/PAS staining分类
农业科技引用本文复制引用
陈茜,韩亮,刘怀东,郭俊辉,孙晓林..天祝白牦牛前后盘吸虫组织结构的观察[J].中国兽医科学,2017,47(9):1106-1111,6.基金项目
国家自然科学基金项目(31460659) （31460659）
甘肃省国际科技合作专项(144WCGA169) （144WCGA169）
