中国兽医科学2017，Vol.47Issue(9)：1118-1123,6.DOI:10.16656/j.issn.1673-4696.2017.09.008
表达猪流行性腹泻病毒S1蛋白的重组乳酸乳球菌的构建及其免疫原性的研究
Construction and immunogenicity of recombinant Lactococcus lactis expressing S1 protein of porcine epidemic diarrhea virus
摘要
Abstract
To evaluate the specific immune responses induced by recombinant Lactococcus lactis(L.lactis) which expresses porcine epidemic diarrhea virus (PEDV) S1 protein as anoral vaccine,the spike gene fragment of PEDV was amplified from the genome and then was linked to the expression vector pMG36e.The recombinant plasmid was electro-transferred into competent L.lactis MG1363.Expression of objective protein was confirmed by SDS-PAGE and Western-blot.New Zealand White rabbits were orally administered with the recombinant strain,then the titer of mucosal immune antibody in intestinal mucosa and IgG in serum was detected by neutralizing test,and the titer of IgG was evaluated by indirect ELISA.The result showed that the high level of IgG in serum and limited mucosal immune antibody could be detected.The recombinant strain is qualified to be a potential oral vaccine because it could successfully evoke both humoral and mucosal immune responses against PEDV.关键词
猪流行性腹泻病毒/S蛋白/pMG36e表达载体/乳酸乳球菌/免疫反应Key words
porcine epidemic diarrhea virus/spike protein/pMG36e vector/Lactococcus lactis MG1363/immune response分类
农业科技引用本文复制引用
王莉平,韩先杰,王孝彬,盖春云,李军伟,单虎..表达猪流行性腹泻病毒S1蛋白的重组乳酸乳球菌的构建及其免疫原性的研究[J].中国兽医科学,2017,47(9):1118-1123,6.基金项目
山东省现代农业产业技术体系项目(SDAIT-06-022-08) （SDAIT-06-022-08）
青岛市民生科技计划项目(16-6-2-42-nsh) （16-6-2-42-nsh）
