中国兽医科学2017，Vol.47Issue(9)：1135-1139,5.DOI:10.16656/j.issn.1673-4696.2017.09.011
鸡烟曲霉菌SYBR Green Ⅰ荧光定量PCR检测方法的建立
Development of SYBR Green Ⅰ real-time PCR for detecting Aspergillus fumigatus in chicken
摘要
Abstract
For the purpose of setting up a convenient,discriminative and sensitive method to detect Aspegillus fumigatus in chicken,according to the benAfum gene sequence of A.fumigatus in GenBank,a pair of special primers were designed.SYBR Green Ⅰ real-time PCR for detecting A.fumigatus in chicken was established.The developed method was used to detect A.fumigatus strains.In result,the method was high sensitive with the lowest detection limit being 10 copies/μL and with a good reproducibility.The variation coefficient ranged from 0.7％ to 0.8％.The methed was highly discriminative without the same reactions with the other pathogenic fungi as Aspergillus flavus,Aspergillus terreus,Aspergillus niger,Aspergillus tubingensis and Candidaalbicans,took less time to complete the test,being 2 h.The above-mentioned results showed that the developed SYBR Green Ⅰ real-time PCR could be applied to clinical detection of A.fumigatus in chicken.关键词
鸡/烟曲霉菌/SYBR Green Ⅰ荧光定量PCRKey words
chicken/Aspergillus fumigatus/SYBR Green Ⅰ real-time PCR分类
农业科技引用本文复制引用
刘利强,沈洁,刘建钗,刘娜,刘彦威,张欣,刘洁玉..鸡烟曲霉菌SYBR Green Ⅰ荧光定量PCR检测方法的建立[J].中国兽医科学,2017,47(9):1135-1139,5.基金项目
河北省科技厅项目(2016D15201) （2016D15201）
