中国兽医科学2017，Vol.47Issue(9)：1150-1153,4.DOI:10.16656/j.issn.1673-4696.2017.09.014
猪嵴病毒重组VP3蛋白间接ELISA检测方法的建立
Establishment of an indirect ELISA based on VP3 protein for the detection of the antibodies against porcine kobuvirus
摘要
Abstract
To establish a serological diagnosis method of porcine kobuvirus(PKV) infection,an indirect ELISA was developed for the detection of PKV antibodies with the VP3 protein as a coating antigen expressed in Escherichia coli.Under the optimized reaction conditions,the assay was able to specifically detect the positive sera against PKV,without cross-reactions with porcine epidemic diarrhea virus,transmissible gastroenteritis virus of swine,or porcine rotavirus,which can cause diarrhea in pigs too.Detection results showed that RT PCR and ELISA were well consistent,which manifested the ELISA was a useful method to investigate the prevalence of PKV.关键词
猪嵴病毒/VP3蛋白/原核表达/间接ELISAKey words
porcine kobuvirus/VP3 protein/prokaryotic expression/indirect ELISA分类
农业科技引用本文复制引用
朱庆贺,苗艳,李文辉,李长龙..猪嵴病毒重组VP3蛋白间接ELISA检测方法的建立[J].中国兽医科学,2017,47(9):1150-1153,4.基金项目
黑龙江省应用技术研究与开发计划项目(sczzn2016-2) （sczzn2016-2）
