中国现代医学杂志2017，Vol.27Issue(21)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.21.001
细胞外信号蛋白调节激酶5在异氟醚后处理减轻大鼠海马脑片缺氧无糖损伤中的作用
Involvement of ERK5 in Isoflurane posttreatment-mediated protective effect on hippocampal oxygen-glucose deprivation injury in rats
摘要
Abstract
Objective To investigate the role of extracellular signal-regulated protein kinase 5 (ERK5) on Isoflurane posttreatment-mediated decrease of hippocampal injury in rat models of oxygen-glucose deprivation (OGD).Methods Male SD rats were randomly divided into four groups:the normal control (Con) group,the OGD group,the XMD group,and the ISPOC group.Rats were anesthetized followed by harvest of the hippocampus for further preparation of tissue slices.Histological analysis of hippocampus was achieved by 2％ 2,3,5-triphenyltetrazolium chloride (TTC) staining.Neuronal apoptosis in hippocampus CA1 region was analyzed by propidium iodide (PI) fluorescence staining.The expression level of ERK5 mRNA was determined by real-time fluorescent PCR.The ERK5 protein expression and phosphorylation were detected by Western blot.Results Compared with the Con group,the degree of hippocampal injury,apoptosis rate in hippocampal CA1 region and the expression levels of ERK5 mRNA and p-ERK5 in the OGD group were increased significantly (P ＜ 0.05).In the XMD group,the expression levels of ERK5 mRNA and p-ERK5 were decreased,and the degree of tissue injury and the apoptosis rate in hippocampal CA1 region increased (P＜ 0.05).Compared with the group 3.0％ ISPOC,XMD8-92 independently diminished the expression of ERK5 mRNA and phosphorylation of ERK5 in the hippocampus,and enhanced tissue injury as well as apoptosis rate in the XMD+ISPOC group (P ＜ 0.05).Conclusions The protective effect of Isoflurane posttreatment on hippocampal OGD injury in rats may be related to the upregulation of ERK5 mRNA and phosphorylation of ERK5.关键词
麻醉药/异氟醚后处理/细胞外信号调节MAP激酶类/脑缺血/海马Key words
anesthetics/Isoflurane posttreatment/extracellular signal-regulated MAP kinases/brain ischemia/hippocampus分类
医药卫生引用本文复制引用
崔迪,王胜,葛明月,王芹,殷姜文,代志刚,司军强..细胞外信号蛋白调节激酶5在异氟醚后处理减轻大鼠海马脑片缺氧无糖损伤中的作用[J].中国现代医学杂志,2017,27(21):1-6,6.基金项目
国家自然科学基金(No:81360203) （No:81360203）
