中国现代医学杂志2017，Vol.27Issue(21)：12-17,6.DOI:10.3969/j.issn.1005-8982.2017.21.003
降钙素基因相关肽对脂多糖诱导血管平滑肌细胞TLR4/NK-κB及炎症因子表达的影响
Effect of CGRP on lipopolysaccharide-induced expressions of TLR4/NK-κB and inflammatory factors of vascular smooth muscle cells
摘要
Abstract
Objective To investigate the effect of calcitonin gene-related peptide (CGRP) on lipopolysaccharide (LPS)-induced expressions of Toll-like receptor 4 (TRL4)/NK-κB and cytokines in vascular smooth muscle cells (VSMCs).Methods VSMCs were cultured and divided randomly into five groups:blank control group,LPS-insulted group,CGRP-stimulated group,LPS+CGRP stimulated group and LPS+CGRP+C8-37 group.Enzyme-linked immunosorbent assay (ELISA) was used to measure concentrations of IL-1β and TNF-α.Western blot was used to detect the expression of TLR4,IκBa and p65.Results LPS upregulated the expressions of IL-1β and TNF-α in a both time-and dose-dependent manner (P＜ 0.05),which reached the peak value when the cells were insulted by LPS at 1,000 ng/ml for 8 hours.CGRP reduced the LPS-induced expressions of IL-1β and TNF-α in a dose-dependent manner,which reached the lowest values when CGRP was under 100 nmol/L (P＜ 0.05).Moreover,LPS-treated cells exhibited significant decrease of TLR4 and phosphorylation of IκBa and p65,which was attenuated significantly by CGRP (P＜ 0.05).As a specific CGRP antagonist,C8-37 reversed the expression levels of IL-1β and TNF-α in VSMCs stimulated by LPS.Conclusions The present study demonstrates that CGRP can significantly block LPS-induced inflammatory response in VSMCs through inhibition of TLR4-mediated NF-κB signaling pathway.关键词
降钙素基因相关肽/血管平滑肌细胞/Toll样受体4/NF-κBKey words
calcitonin gene-related peptide/vascular smooth muscle cell/Toll-like receptor 4/NK-κB分类
医药卫生引用本文复制引用
王芳,周祥群,付洁..降钙素基因相关肽对脂多糖诱导血管平滑肌细胞TLR4/NK-κB及炎症因子表达的影响[J].中国现代医学杂志,2017,27(21):12-17,6.基金项目
海南省卫生厅课题(No:1421320.24A1004) （No:1421320.24A1004）
