中国现代医学杂志2017，Vol.27Issue(21)：18-24,7.DOI:10.3969/j.issn.1005-8982.2017.21.004
啤酒酵母Ubr1野生型和缺失型菌株的比较蛋白组学分析
Comparative proteomic analysis of Ubr1 wild type and deletion type of Saccharomyces cerevisiae
摘要
Abstract
Objective To justify the rationality of proteomic screening on a large scale and identify potential substrates of Ubr1 with the approach.Methods In this work,comparative proteomic analysis between RJD347 (Ubr1 wild type) and AVY26 (Ubr1 deletion type) has been performed to screen the possible substrate proteins of Ubrl.Moreover,the correlation between the differentially expressed proteins and Ubr1 was further verified by protein work.Results In total,249 proteins which were differentially expressed were identified,of which 145 proteins were up-regulated in AVY26.Furthermore,the protein work confirmed 5 proteins including MLC2,SCD6,ARG1,HOG1 and RTF1 that were closely related to Ubr1.Conclusions A massive database of 249 proteins that are differentially expressed between RJD347 and AVY26 is established.Five potential substrates of Ubr1 ubiquitination proteins is identified,which provides the experimental basis for further understanding of biological processes of Ubr1.关键词
啤酒酵母/泛素连接酶E3成分N-识别蛋白1/泛素化/蛋白质组学Key words
Saccharomyces cerevisiae/ubiquitin protein ligase E3 component N-recognin 1/ubiquitination/proteomics分类
生物科学引用本文复制引用
彭健,陈杰,丁成明,夏赞贤,张耀婷,李慧,肖小龙,韦超盈,周宇筝,卢爱,张礼铭,周梅..啤酒酵母Ubr1野生型和缺失型菌株的比较蛋白组学分析[J].中国现代医学杂志,2017,27(21):18-24,7.基金项目
国家自然科学基金联合项目(No:U1603126) （No:U1603126）
湖南省自然科学基金(No:2017JJ2334) （No:2017JJ2334）
