中国现代医学杂志2017，Vol.27Issue(21)：25-30,6.DOI:10.3969/j.issn.1005-8982.2017.21.005
凝溶胶蛋白对胃癌细胞凋亡及细胞中活性氧水平的影响研究
Effect of GSN on apoptosis and ROS level in gastric cancer cells
摘要
Abstract
Objective To investigate the effect of GSN on the apoptosis and the level of ROS in gastric cancer cells.Methods The expression of GSN in gastric cancer cell lines SGC7901,AGS,BGC823 and gastric epithelial cells GES-1 were detected by Western blot.Cells were transfected with GSN siRNA (the GSN siRNA group),siRNA control (the siRNA control group) and transfection vehicle (the vehicle control group),respectively.Cell proliferation was determined by MTT assay.Cell apoptosis was determined by flow cytometry.ROS level was measured by laser scanning confocal microscopy.The levels of GSN,NICD1,NICD2,HES1,and cleaved Caspase-3 were detected by Western blot.Results Expression level of GSN in gastric cancer cell lines including SGC7901,AGS and BGC823 was significantly higher than that in gastric epithelial cells GES-1 (P ＜ 0.05).Given that AGS witnessed the highest expression of GSN,following experiments were performed on it.There was no significant difference in cell proliferation,apoptosis,ROS,GSN,NICD1,NICD2,HES1 or cleaved Caspase-3 level between the siRNA control group and the vehicle control group (P＞ 0.05).Cell proliferation and the levels of GSN,NICD1,NICD2,and HES1 in the GSN siRNA group were dramatically lower than those in the control group (P＜ 0.05),while cell apoptosis and the levels of ROS and cleaved Caspase-3 were notably higher than those in the vehicle control group (P ＜ 0.05).Conclusions GSN decreases proliferation ability while increases apoptosis of gastric cancer cells through manipulation on ROS level and NOTCH signaling pathway.关键词
胃癌细胞/凋亡/NOTCH信号通路/活性氧Key words
gastric cancer cell/apoptosis/NOTCH signaling pathway/reactive oxygen specie分类
医药卫生引用本文复制引用
倪猛,殷涛,郑喜胜,刘驰,王博,樊宏伟..凝溶胶蛋白对胃癌细胞凋亡及细胞中活性氧水平的影响研究[J].中国现代医学杂志,2017,27(21):25-30,6.基金项目
国家自然科学基金(No:81372665) （No:81372665）
