中国现代医学杂志2017，Vol.27Issue(21)：37-42,6.DOI:10.3969/j.issn.1005-8982.2017.21.007
脂联素抑制人髓核细胞分泌疼痛介质PGE2的初步机制研究
Inhibitory effect of adiponectin on secretion of PGE2 in human nucleus pulposus cells
梁志白 1赵枫1
作者信息
- 1. 中国人民解放军第180医院骨二科,福建泉州362000
- 折叠
摘要
Abstract
Objective To investigate the anti-inflammatory effects of adiponectin in patients with discogenic back pain and the potential mechanism.Methods The expression of adiponectin receptor Adipo R1/2 in human nucleus pulposus (NP) tissue was examined.Human NP cells were isolated for further culture.The regulatory effect of TNF-α on adiponectin receptors in NP cells was detected.Moreover,lentiviral gene silencing technology was utilized to investigate the inhibitory effect of adiponectin on TNF-α-induced production of PGE-2 and underlying mechanism.Results The expression of Adipo R1/2 was significantly decreased in NP tissues derived from patients with discogenic back pain compared with normal NP tissues (P ＜ 0.05).TNF-α inhibited the expression of Adipo R1/2,and up-regulated the secretion of PGE2 in NP cells,which was attenuated by adiponectin dose-dependently (P＜ 0.05).Over-expression of Adipo R1/2 abolished the TNF-α-induced PGE2 production (P＜ 0.05).Conclusions Adiponectin is potentially a protective mediator in patients with discogenic back pain through AdipoR1/2-dependent inhibition of secretion of PGE2.关键词
椎间盘源性腰痛/脂联素/炎症反应/PGE-2Key words
discogenic back pain/adiponectin/inflammation/PGE2分类
医药卫生引用本文复制引用
梁志白,赵枫..脂联素抑制人髓核细胞分泌疼痛介质PGE2的初步机制研究[J].中国现代医学杂志,2017,27(21):37-42,6.
