中国现代医学杂志2017，Vol.27Issue(21)：66-70,5.DOI:10.3969/j.issn.1005-8982.2017.21.012
焦磷酸测序法和Sanger测序法检测丙型肝炎病毒基因分型的方法学比较
Comparsion of Pyrosequencing and Sanger sequencing in detection of hepatitis C virus genotypes
渠滕 1田文君 1刘义庆 1刘春梅 1张庆 1崔朝杰 1邱旸1
作者信息
- 1. 山东大学附属省立医院临床检验医学部,山东济南250021
- 折叠
摘要
Abstract
Objective To compare the accuracy and sensitivity of Pyrosequencing and Sanger sequencing in detection of hepatitis C virus genotypes.Methods Pyrosequencing and Sanger sequencing were used to detect the hepatitis C virus genotypes using plasma of the same 100 patients,results of the two methods were analyzed.Results The results were consistent in 92 of 100 cases when detecting the HCV-RNA genotyping,of which the concordance rate was 92％.There were 80 samples whose HCV-RNA concentration was above 1 ×104 IU/ml,with completely consistent sequencing results of the two methods.And there were 20 samples whose HCV-RNA concentration was below 1 ×104 IU/ml,all of which were detected successfully by Pyrosequencing,while 14 of which were detected successfully by Sanger sequencing,12 of which had identical results.Conclusions Compared with Sanger sequencing,Pyrosequencing can make results accurate and reliable,and the sensitivity of which is higher.In addition,Pyrosequencing can be used for quantitative analysis.关键词
丙型肝炎/焦磷酸测序/Sanger测序Key words
hepatitis C/Pyrosequencing/Sanger sequencing分类
医药卫生引用本文复制引用
渠滕,田文君,刘义庆,刘春梅,张庆,崔朝杰,邱旸..焦磷酸测序法和Sanger测序法检测丙型肝炎病毒基因分型的方法学比较[J].中国现代医学杂志,2017,27(21):66-70,5.
