中国药房2017，Vol.28Issue(27)：3828-3831,4.DOI:10.6039/j.issn.1001-0408.2017.27.23
脑得生固体分散体胶囊的HPLC指纹图谱研究
Study on HPLC Fingerprints of Naodesheng Solid Dispersion Capsules
摘要
Abstract
OBJECTIVE:To establish HPLC fmgerprints of Naodesheng solid dispersion capsules.METHODS:HPLC method was adopted.The determination was performed on Hyspersil ODS2 column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelengths were 210 nm (puerarin),345 nm (hydroxfsaffior yellow A).The column temperature was 30 ℃,and sample size was 10 μL.Using puerarin and hydroxysaffior yellow A as reference,HPLC fingerprints of 10 batches of sample were determined.Similarity evaluation,common peak identification and chemical components confirmation were performed by using Similarity Evaluation System of TCM Chromatographic Fingerprints (2012 edition).RESULTS:There were 29 and 23 common peaks in HPLC fingerprints of 10 batches of samples at 210 nm and 345 nm,respectively.The similarity was higher than 0.90.The medicinal material attribute of common peaks were Panax notoginseng,Ligusticum chuanxiong,Carthamus tinctorius,Pueraia lobata and Crataegus pinnatifida.Moreover,7 chemical components were identified at 210,345 nm,respectively.CONCLUSIONS:Established HPLC fingerprints can provide reference for identification and quality evaluation of Naodesheng solid dispersion capsules.关键词
脑得生固体分散体胶囊/高效液相色谱法/指纹图谱Key words
Naodesheng solid dispersion capsules/HPLC/Fingerprint分类
医药卫生引用本文复制引用
罗兰,李明丽,康家珍,王淑美,梁生旺..脑得生固体分散体胶囊的HPLC指纹图谱研究[J].中国药房,2017,28(27):3828-3831,4.基金项目
国家自然科学基金资助项目(No.81274059) （No.81274059）
