中国药房2017，Vol.28Issue(27)：3832-3835,4.DOI:10.6039/j.issn.1001-0408.2017.27.24
复方守宫散的质量标准提高研究
Study on the Improvement of Quality Standard of Compound Shougong Powder
摘要
Abstract
OBJECTIVE:To improve the quality standard of Compound shougong powder.METHODS:Gekko japonicas and Armeniaca mume were identified by microscopic identification.TLC method was adopted to identify processed Fallopia multiflora qualitatively.The content of 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside (stilbene glycoside) was determined by HPLC.The determination was performed on Agilent Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water (19 ∶ 81,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 320 nm,and column temperature was 25 ℃.The sample size was 10 μL.RESULTS:Microscopic characteristics of skin fragments and scales of G.japonicas and the pollen grains of A.mume were significant without interference from negative control.TLC spots of F.multiflora were clear and well-separated without interference from negative control.The linear range of stilbene glucoside were 23-368 μg/mL (r=0.999 9).RSDs of precision,stability and reproducibility tests were all lower than 1.0％.The recoveries ranged 98.69％-101.61％ (RSD=0.94％,n =9).CONCLUSIONS:Improved standard can effectively control the quality of Compound shougong powder.关键词
复方守宫散/显微鉴别法/薄层色谱法/含量测定/高效液相色谱法Key words
Compound shougong powder/Microscopy identification method/TLC/Content determination/HPLC分类
医药卫生引用本文复制引用
王晓玉,孟楣,汪永忠..复方守宫散的质量标准提高研究[J].中国药房,2017,28(27):3832-3835,4.基金项目
国家科技支撑计划新安医学传承与发展研究项目(No.2012BAI26B03) （No.2012BAI26B03）
