中国药房2017，Vol.28Issue(27)：3836-3838,3.DOI:10.6039/j.issn.1001-0408.2017.27.25
HPLC法同时测定五味沙棘颗粒中甘草苷和甘草酸的含量
Simultaneous Determination of Liquiritin and Glycyrrhizic Acid in Wuwei Shaji Granules by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules.METHODS:HPLC method was adopted.The determination was performed on Thermo scientific ODS-2 Hypersil column with mobile phase consisted of acetonitrile-0.5％ phosphoric acid (gradient elution) with flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 25 ℃.The sample size was 20 μL.RESULTS:The linear ranges of liquiritin and glycyrrhizic acid were 48-480 μg(r=0.999 9) and 90-900 μg(r=0.999 9),respectively.The limits of quantitation were 0.55,2.10 μg/mL,and the limits of detection were 0.16,0.62 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 95.08％-97.58％ (RSD=0.93％,n=6)and 95.86％-99.89％ (RSD=1.67％,n=6),respectively.CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the content determination of liquiritin and glycyrrhizic acid in Wuwei shaji granules.关键词
高效液相色谱法/五味沙棘颗粒/甘草苷/甘草酸/含量测定Key words
HPLC/Wuwei shaji granules/Liquiritin/Glycyrrhizic acid/Content determination分类
医药卫生引用本文复制引用
耿婕,那顺朝克图,丛丽华,樊精辉,赵春雨,薛培凤..HPLC法同时测定五味沙棘颗粒中甘草苷和甘草酸的含量[J].中国药房,2017,28(27):3836-3838,3.基金项目
国家科技支撑计划项目(No.2011BAI07B02) （No.2011BAI07B02）
内蒙古自治区“草原英才”项目(No.内组通字[2014]27号) （No.内组通字[2014]27号）
