中国药房2017，Vol.28Issue(27)：3839-3842,4.DOI:10.6039/j.issn.1001-0408.2017.27.26
炮山甲的HPLC指纹图谱研究
Study on HPLC Fingerprint of Stir-baked Manis pentadactyla
摘要
Abstract
OBJECTIVE:To establish HPLC fingerprint of stir-baked Manis pentadactyla.METHODS:HPLC method was conducted.The determination was performed on Capcell Pak Mg Ⅱ S5 C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL/min.The detection wavelength was set at 275 nm,and column temperature was 30 ℃.The sample size was 10 μL.Using oxyphenylaminopropionic acid as reference,HPLC chromatograms of 11 batches of medicinal materials were determined.TCM Chromatogram Fingerprint Similarity Evaluation System (2004 A edition) was used for common peak identification and similarity evaluation.RESULTS:There were 23 common peaks in 11 batches of stir-baked M.pentadactyla,with similarity＞0.90.After validation,HPLC chromatograms of 11 batches of medicinal materials were in good agreement with control fingerprints.CONCLUSIONS:Established HPLC fingerprint can provide reference for the identification and quality evaluation of stir-baked M.pentadactyla.关键词
炮山甲/高效液相色谱法/指纹图谱/质量评价Key words
Stir-baked Manis pentadactyla/HPLC/Fingerprint/Quality evaluation分类
医药卫生引用本文复制引用
严丹,刘玉杰,胡美变,肖禾,李永川,吴纯洁..炮山甲的HPLC指纹图谱研究[J].中国药房,2017,28(27):3839-3842,4.基金项目
国家中医药行业科研专项项目(No.201207004-6) （No.201207004-6）
