中国肿瘤生物治疗杂志2017，Vol.24Issue(9)：938-943,6.DOI:10.3872/j.issn.1007-385x.2017.09.002
EBV-LMP2A肽诱导的特异性CTL对EBV阳性胃癌细胞的体外杀伤作用
Cytotoxicity of specific CTL induced by EBV-LMP2A peptide on human EBV associated gastric carcinoma cell in vitro
摘要
Abstract
Objective:To study the cytotoxicity of specific cytotoxic T lymphocyte (CTL) induced by EBVLPM2A peptide on human EBV associated gastric cancer cells in vitro.Methods:The peripheral blood mononuclear cells (PBMC) of HLA-A2 positive gastric cancer patients from Cancer Center of Drum Tower Hospital Affiliated to Medical School of Nanjing University,human gastric adenocarcinoma cell line (AGS) and human EBV positive adenocarcinoma cell line (AGS-EBV) were selected.By modified in vitro cell culture technique,specific CTL was amplified from human PBMCs with the induction of HLA-A2 restricted EBV-LMP2A peptide;the content of specific CTL induced by peptide was detected by the method of tetramer and Flow cytometry;the in vitro cytotoxicity of EBV-CTL on both EBV+ and EBV human gastric cancer cells was detected by FITC-PI.Results:Modified cell culture technique and EBV-LMP2A peptide can induce a high proportion of antigen specific CTL (EBV-LMP2A-356 specific T cells accounted for [47.1 ±5.2]％ of CD8+T cells);the cytotoxicity of EBV-CTL on EBV+ gastric cancer cells was significantly stronger than that of EBV gastric cancer cells ([45.1 ± 9.3]％ vs [19.4± 2.5]％,P＜0.05).Conclusion:Using the modified cell culture technique,EBV-LMP2A antigen peptide can induce a high proportion of EBV specific CTL,which has high specific cytotoxicity on EBV+ gastric cancer cells.关键词
EB病毒-潜伏膜蛋白2A/细胞毒性T淋巴细胞/EB病毒阳性胃癌/细胞毒作用Key words
EBV-latent membrane protein 2A(EBV-LMP2A)/cytolytic T lymphocyte (CTL)/EBV-associated gastric carcinoma(EBVaGC)/cytotoxicity分类
医药卫生引用本文复制引用
杜娟,苏舒,孟凡岩,邵洁,徐秋萍,邹征云,刘宝瑞..EBV-LMP2A肽诱导的特异性CTL对EBV阳性胃癌细胞的体外杀伤作用[J].中国肿瘤生物治疗杂志,2017,24(9):938-943,6.基金项目
国家自然科学基金资助项目(No.81602077,No.81572601).Project supported by the National Natural Science Foundation of China (No.81602077,No.81572601) （No.81602077,No.81572601）
