中国肿瘤生物治疗杂志2017，Vol.24Issue(9)：944-949,6.DOI:10.3872/j.issn.1007-385x.2017.09.003
miR-223通过Lmo2基因和MAPK信号通路调控急性淋巴细胞白血病的恶性生物学行为
miR-223 regulates malignant biological behavior of acute lymphoblastic leukemia cells through targeting Lmo2 gene and MAPK signal pathway
陈丽 1赵红勉1
作者信息
- 1. 河南大学淮河医院血液科,河南开封475000
- 折叠
摘要
Abstract
Objective:To investigate the effect and mechanism of miR-223 regulating the proliferation and in vivo tumorigenesis of acute lymphoblastic leukemia (ALL) E6-1 cells.Methods:The expression of miR-223 in different ALL cell lines was detected by Real-time fluorescence quantitative PCR.Immunofluorescence was used to detect the transfection efficiency of miR-223mimic-lenitvirus transfected E6-1 cell line.Double luciferase assay was used to detect the binding between miR-223 and Lmo2.MTT assay and clone formation assay were used to detect the effect of miR-223 on the proliferation and clone formation ability of E6-1 cell line.The effect of miR-223 expression on the tumorigenic ability of E6-1 cells was detected by xenograft formation in nude mice.Western blotting was used to detect the effect of miR-223 on the expressions of MAPK signal pathway related proteins.Results:The expression level ofmiR-223 in E6-1 cell line was relatively low (P＜0.05).Double luciferase assay confirmed that miR223 could directly target the 3'UTR ofLmo2.Overexpression of miR-223 inhibited the proliferation (0.16 ± 0.02 vs 1.15 ± 0.21,P＜0.05) and tumorigenesis ([0.56 ± 0.08]g vs [1.69± 0.22]g,P＜ 0.05) of E6-1 cells and regulated the activity of MAPK signal pathway.Conclusion:miR-223 can regulate the proliferation,clone formation and in vivo tumorigenesis of ALL cells through targeting Lmo2 and MAPK signal pathway.关键词
miRNA-133/Lmo2基因/急性淋巴细胞白血病/E6-1细胞/MAPK信号通路Key words
miR-223/Lmo2 gene/acute lymphoblastic leukemia/E6-1 cell line/MAPK signal pathway分类
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陈丽,赵红勉..miR-223通过Lmo2基因和MAPK信号通路调控急性淋巴细胞白血病的恶性生物学行为[J].中国肿瘤生物治疗杂志,2017,24(9):944-949,6.
