中国肿瘤生物治疗杂志2017，Vol.24Issue(9)：950-954,5.DOI:10.3872/j.issn.1007-385x.2017.09.004
Toll样受体-4表达对树突状细胞表型及功能的影响
Effect of TLR-4 on phenotypes and function of dendritic cells
摘要
Abstract
Objective:To investigate whether the expression of Toll like receptor-4 (TLR-4) has an effect on the phenotypes and function of dendritic cells (DCs) and the possible mechanism.Methods:pcDNA3.1 plasmid encoding full TLR-4 gene sequence was constructed as a template to obtain TLR-4 mRNA through in vitro transcription using mMESSAGE mMACHINE T7 Kit;DCs from healthy human peripheral blood were transfected with TLR-4 mRNA by liposomal transfection.The functional molecule expression on DC surface and the ability of DC inducing cytotoxic T lymphocytes (CTLs) to secrete cytokines in vitro were detected by Flow cytometry after transfection.Results:Human TLR-4-pcDNA3.1 plasmid was successfully constructed;human TLR-4 and EGFP mRNA fragments were successfully amplified.The Flow cytometry results showed that the expressions of chemokine CCR7 and functional molecule HLA-DR on dendritic cell surface were significantly increased after transfection with TLR4 mRNA compared with control mRNA transfection or pre-transfection (CCR7:[42.4±4.93]％ vs [20.1 ±3.09]％,[17.1±4.33]％,P＜0.05;HLA-DR:[62.1±7.23]％ vs [17.7±6.01]％,[25.8±4.16]％,P＜0.05);and the ability of secreting IFN-γ from CTLs induced by TLR-4 mRNA-DCS was significantly enhanced in vitro compared with control mRNA-DC,empty-DC and PBMC ([66.5±3.58]％ vs [41.1±4.27]％,[37.9±2.96]％,[3.2±2.03]％,P＜0.05).Conclusion:The function of dendritic cells could be significantly enhanced by TLR-4 mRNA transfection.The ability of antigen presentation and inducing CTLs of dendritic cell were improved,which would provide an experimental basis for enhancing the anti-tumor effect of DC vaccines.关键词
树突状细胞/细胞毒性T淋巴细胞/Toll样受体-4/抗原提呈/免疫应答Key words
dendritic cell/cytotoxic T lymphocyte(CTL)/Toll-like receptor-4(TLR4)/antigen presenting/immune response分类
医药卫生引用本文复制引用
谢云青,栗世铀,刘施佳,黄丽洁,郑秋红..Toll样受体-4表达对树突状细胞表型及功能的影响[J].中国肿瘤生物治疗杂志,2017,24(9):950-954,5.基金项目
国家临床重点专科建设项目资助 （）
福建省科技计划项目资助(No.2017Y0022) （No.2017Y0022）
福建省自然基金项目资助(No.2016J01514).Project supported by the National Clinical Key Specialty Construction Program,the Fujian Science and Technology Plan (No.2017Y0022),and the Natural Science Foundation of Fujian Province (No.2016J01514) （No.2016J01514）
